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首页> 外文期刊>The Journal of Reproduction and Development >Expression and distribution of the zinc finger protein, SNAI3, in mouse ovaries and pre-implantation embryos
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Expression and distribution of the zinc finger protein, SNAI3, in mouse ovaries and pre-implantation embryos

机译：锌指蛋白SNAI3在小鼠卵巢和植入前胚胎中的表达和分布

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The Snail gene family includes Snai1 , Snai2 , and Snai3 that encode zinc finger-containing transcriptional repressors in mammals. The expression and localization of SNAI1 and SNAI2 have been studied extensively during folliculogenesis, ovulation, luteinization, and embryogenesis in mice. However, the role of SNAI3 is unknown. In this study, we investigated the expression of SNAI3 during these processes. Our immunohistochemistry data showed that SNAI3 first appeared in oocytes by postnatal day (PD) 9. Following this, SNAI3 was found to be expressed consistently in theca and interstitial cells, along with oocytes. In gonadotropin-treated immature mice, the expression of SNAI3 did not change significantly during follicular development. The expression of SNAI3 was reduced during ovulation, after which it increased gradually during luteinization. Similar results were obtained from western blot analyses. Furthermore, real-time polymerase chain reaction (RT-PCR) analyses revealed varying mRNA levels of different Snail factors at a given time in gonadotropin-induced ovaries. During early embryo cleavage, SNAI3 was localized to the nucleus, except the nucleolus at the germinal vesicle and one-cell stages. From two- to eight-cell stages, SNAI3 was localized only to the nucleolus. Thereafter, SNAI3 was detected only in the cytoplasm, except during the blastocyst stage when it was localized to the nucleus of the trophectoderm and the inner cell mass. RT-PCR results showed that the expression of Snail superfamily genes was decreased during the blastocyst stage. From the eight-cell to morula stage, when compaction occurs that is a prerequisite for blastocyst formation, Snai3 mRNA was expressed at very low levels and was opposite to the highest expression level of the compaction-related gene, E-cadherin, at the eight-cell stage. Taken together, our results suggest that SNAI3 likely plays some roles during folliculogenesis, luteinization, and early embryonic development.
机译：Snail基因家族包括Snai1，Snai2和Snai3，它们在哺乳动物中编码含锌指的转录阻遏物。 SNAI1和SNAI2的表达和定位已经在小鼠的卵泡形成，排卵，黄体生成和胚胎发生过程中进行了广泛的研究。但是，SNAI3的作用尚不清楚。在这项研究中，我们调查了这些过程中SNAI3的表达。我们的免疫组织化学数据显示，SNAI3在出生后第9天首次出现在卵母细胞中。此后，发现SNAI3在卵母细胞和间质细胞中与卵母细胞一致表达。在促性腺激素治疗的未成熟小鼠中，SNAI3的表达在卵泡发育过程中没有显着变化。 SNAI3的表达在排卵过程中减少，此后在黄体化过程中逐渐增加。从蛋白质印迹分析获得了相似的结果。此外，实时聚合酶链反应（RT-PCR）分析显示，促性腺激素诱导的卵巢在给定的时间不同Snail因子的mRNA水平有所变化。在早期卵裂期间，SNAI3定位于细胞核，除了在生小泡和单细胞阶段的核仁。从2到8个细胞阶段，SNAI3仅定位于核仁。此后，仅在胞浆中检测到SNAI3，除了在胚泡阶段定位在滋养外胚层的核和内部细胞团期间。 RT-PCR结果表明，在囊胚期Snail超家族基因的表达降低。从八细胞到桑ula虫阶段，当紧致发生成为胚泡形成的先决条件时，Snai3 mRNA的表达水平非常低，与紧实相关基因E-钙粘着蛋白的最高表达水平相反。 -细胞阶段。两者合计，我们的结果表明SNAI3可能在卵泡形成，黄体化和早期胚胎发育过程中发挥某些作用。

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