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首页> 外文期刊>The Journal of Reproduction and Development >Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries
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Expression and Function of Apoptosis Initiator FOXO3 in Granulosa Cells During Follicular Atresia in Pig Ovaries

机译：猪卵巢滤泡闭锁过程中颗粒细胞凋亡启动子FOXO3的表达及功能

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In mammalian ovaries, most follicles are lost by atresia before ovulation. It has become apparent that the apoptosis of granulosa cells induces follicular atresia. Forkhead box O3 (FOXO3), also called FKHRL1 (forkhead in rhabdomyosarcoma-like 1), is a proapoptotic molecule that belongs to the FOXO subfamily of forkhead transcription factors. Foxo3 -deficient female mice were reported to be infertile because of abnormal ovarian follicular development, but the precise influences of FOXO3 on follicular atresia of mature ovary have not been determined. Therefore, we examined the expression and function of FOXO3 in porcine ovarian follicles and granulosa-derived cells. FOXO3 mRNA levels in granulosa cells of porcine ovaries increased during atresia, while FOXO3 protein was abundant in granulosa cells of early atretic follicles. By immunohistochemistry, the inner surface area of the granulosa layer in early atretic follicles was strongly stained with anti-FOXO3 antibody. The granulosa cells expressing FOXO3 coincided with apoptotic cells, indicating a role of FOXO3 as a proapoptotic factor in granulosa cells of porcine ovaries. In porcine (JC-410) and human (KGN) granulosa-derived cells, cell death was induced by transfection of FOXO3 expression vectors. Expression of the proapoptotic factors Fas ligand (FASLG) and BCL2-like 11 (BCL2L11) was upregulated by FOXO3 in KGN cells. In conclusion, FOXO3 is expressed in porcine ovarian follicles and induces apoptosis in granulosa cells, suggesting that it is a candidate for the initiator of follicular atresia.
机译：在哺乳动物卵巢中，大多数卵泡在排卵前因闭锁而丢失。显而易见的是，颗粒细胞的凋亡诱导滤泡闭锁。叉头盒O3（FOXO3），也称为FKHRL1（横纹肌肉瘤样1中的叉头），是一种促凋亡分子，属于叉头转录因子的FOXO亚家族。据报道，缺乏Foxo3的雌性小鼠由于卵巢卵泡发育异常而无法生育，但尚未确定FOXO3对成熟卵巢的卵泡闭锁的确切影响。因此，我们检查了FOXO3在猪卵巢卵泡和颗粒细胞中的表达和功能。在闭锁期间，猪卵巢颗粒细胞中的FOXO3 mRNA水平升高，而早期闭锁卵泡的颗粒细胞中FOXO3蛋白丰富。通过免疫组织化学，早期FOCO3抗体对早期闭锁卵泡中颗粒层的内表面进行了强烈染色。表达FOXO3的颗粒细胞与凋亡细胞重合，表明FOXO3作为猪卵巢颗粒细胞中的促凋亡因子。在猪（JC-410）和人（KGN）颗粒来源的细胞中，通过转染FOXO3表达载体诱导细胞死亡。 FOXO3在KGN细胞中上调凋亡因子Fas配体（FASLG）和BCL2样11（BCL2L11）的表达。总之，FOXO3在猪卵巢卵泡中表达，并诱导颗粒细胞凋亡，表明它是卵泡闭锁性启动子的候选者。

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