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首页> 外文期刊>The Journal of Reproduction and Development >Unstable Side Population Phenotype of Mouse Spermatogonial Stem Cells In Vitro
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Unstable Side Population Phenotype of Mouse Spermatogonial Stem Cells In Vitro

机译：小鼠精原干细胞的不稳定侧种群表型

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Stem cells of the side population (SP) phenotype are found in many self-renewing tissues and can be identified by their unique ability to effectively exclude the dye Hoechst 33342. We previously established a method for expanding spermatogonial stem cells (SSCs) in vitro , but the frequency of SSCs is only about 1 to 2%, limiting detailed SSC analyses. In this study, we sought to isolate SSCs from in vitro cultures by exploiting their ability to exclude Hoechst 33342. In contrast to the findings of previous in vivo studies, we found that SP cells developed in a stochastic manner in vitro . Moreover, SP cells in culture were not enriched in SSCs, but they were interconvertible with non-SP cells. Although SP cells were consistently found in testes after transplantation of cultured cells, they were not enriched in SSCs. These results show that SSCs have an unstable SP phenotype and provide evidence that SSCs change their phenotype characteristics in response to their microenvironment.
机译：在许多自我更新的组织中发现了侧群（SP）表型的干细胞，并且可以通过其有效排除染料Hoechst 33342的独特能力进行鉴定。我们先前建立了一种体外扩增精原干细胞（SSC）的方法，但是SSC的频率仅为1-2％，这限制了详细的SSC分析。在这项研究中，我们试图通过利用SSC排斥Hoechst 33342的能力从体外培养物中分离SSC。与先前的体内研究发现相反，我们发现SP细胞在体外以随机方式发育。此外，培养中的SP细胞未富集SSC，但可与非SP细胞互换。尽管在培养细胞移植后始终在睾丸中发现了SP细胞，但它们并未富含SSC。这些结果表明，SSC具有不稳定的SP表型，并提供证据表明SSC响应其微环境而改变其表型特征。

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