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首页> 外文期刊>The Journal of Reproduction and Development >Non-CpG Methylation Occurs in the Regulatory Region of the Sry Gene
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Non-CpG Methylation Occurs in the Regulatory Region of the Sry Gene

机译：非CpG甲基化发生在Sry基因的调控区域。

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The Sry (sex determining region on Y chromosome) gene is a master gene for sex determination. We previously reported that the Sry gene has tissue-dependent and differentially methylated regions (T-DMRs) by analyzing the DNA methylation states at CpG sites in the promoter regions. In this study, we found unique non-CpG methylation at the internal cytosine in the 5'-CCTGG-3' pentanucleotide sequence in the Sry T-DMR. This non-CpG methylation was detected in four mouse strains (ICR, BALB/c, DBA2 and C3H), but not in two strains (C57BL/6 and 129S1), suggesting that the CCTGG methylation is tentative and unstable. Interestingly, this CCTGG methylation was associated with demethylation of the CpG sites in the Sry T-DMR in the developmental process. A methylation-mediated promoter assay showed that the CCTGG methylation promotes gene expression. Our finding shows that non-CpG methylation has unique characteristic and is still conserved in mammals.
机译：Sry（Y染色体上的性别决定区域）基因是性别决定的主要基因。通过分析启动子区域CpG位点的DNA甲基化状态，我们先前报道了Sry基因具有组织依赖性和甲基化差异区域（T-DMR）。在这项研究中，我们在Sry T-​​DMR的5'-CCTGG-3'五核苷酸序列的内部胞嘧啶中发现了独特的非CpG甲基化。在四个小鼠品系（ICR，BALB / c，DBA2和C3H）中检测到了这种非CpG甲基化，但在两个品系（C57BL / 6和129S1）中未检测到，这表明CCTGG甲基化是暂时的且不稳定的。有趣的是，这种CCTGG甲基化与发育过程中Sry T-​​DMR中CpG位点的脱甲基有关。甲基化介导的启动子测定表明CCTGG甲基化促进基因表达。我们的发现表明，非CpG甲基化具有独特的特征，并且在哺乳动物中仍然保守。

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