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首页> 外文期刊>The Journal of Reproduction and Development >Ploidy Assessment of Porcine Haploid and Diploid Parthenogenetic Embryos by Fluorescent In Situ Hybridization Detecting a Chromosome 1-Specific Sequence, Sus scrofa Mc1 Satellite DNA
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Ploidy Assessment of Porcine Haploid and Diploid Parthenogenetic Embryos by Fluorescent In Situ Hybridization Detecting a Chromosome 1-Specific Sequence, Sus scrofa Mc1 Satellite DNA

机译：荧光原位杂交检测染色体1特定序列，Sus scrofa Mc1卫星DNA的猪单倍体和二倍体孤雌生殖胚的倍性评估

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摘要

The aim of the present study was to examine the feasibility of fluorescent in situ hybridization (FISH) for detecting a chromosome 1-specific sequence as a means of assessing the ploidy of porcine parthenotes. In vitro -matured oocytes with the first polar body (PB) were electrically activated; some were treated with cytochalasin B to prevent second PB extrusion (1PB embryos), and the others extruded the second PB (2PB embryos). At the 2-cell stage, one and two FISH signals were detected in each nucleus of 2PB and 1PB embryos, respectively. Almost all cells of blastocysts derived from 1PB embryos retained two signals. In contrast, cells of blastocysts derived from 2PB embryos had two signals. These data demonstrate that FISH analysis allows precise ploidy assessment of porcine parthenogenetic embryos, hence providing a practical means of detecting ploidy transition during parthenogenetic embryogenesis.
机译：本研究的目的是检验荧光原位杂交（FISH）检测1号染色体特异性序列作为评估猪单性生殖倍性的可行性。具有第一极体（PB）的体外成熟卵母细胞被电激活。一些用细胞松弛素B进行处理以防止第二个PB挤出（1PB胚胎），而另一些用第二个PB挤出（2PB胚胎）。在2细胞阶段，分别在2PB和1PB胚胎的每个核中检测到一个和两个FISH信号。几乎所有源自1PB胚胎的胚泡细胞都保留了两个信号。相反，源自2PB胚胎的胚泡细胞具有两个信号。这些数据表明，FISH分析可对猪孤雌生殖胚胎进行精确的倍性评估，从而为检测孤雌生殖胚胎发生过程中的倍性转变提供了一种实用的手段。

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