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首页> 外文期刊>The Journal of Reproduction and Development >Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2’-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos
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Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2’-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos

机译：用5-氮杂-2'-脱氧胞苷处理克隆的胚胎，但不能处理供体细胞，可增强猪克隆胚胎的发育能力。

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摘要

The efficiency of cloning by somatic cell nuclear transfer (SCNT) has remained low. In most cloned embryos, epigenetic reprogramming is incomplete, and usually the genome is hypermethylated. The DNA methylation inhibitor 5-aza-2’-deoxycytidine (5-aza-dC) could improve the developmental competence of cow, pig, cat and human SCNT embryos in previous studies. However, the parameters of 5-aza-dC treatment among species are different, and whether 5-aza-dC could enhance the developmental competence of porcine cloned embryos has still not been well studied. Therefore, in this study, we treated porcine fetal fibroblasts (PFF) that then were used as donor nuclei for nuclear transfer or fibroblast-derived reconstructed embryos with 5-aza-dC, and the concentration- and time-dependent effects of 5-aza-dC on porcine cloned embryos were investigated by assessing pseudo-pronucleus formation, developmental potential and pluripotent gene expression of these reconstructed embryos. Our results showed that 5-aza-dC significantly reduced the DNA methylation level in PFF (0 nM vs. 10 nM vs. 25 nM vs. 50 nM, 58.70% vs. 37.37% vs. 45.43% vs. 39.53%, P
机译：通过体细胞核转移（SCNT）进行克隆的效率仍然很低。在大多数克隆的胚胎中，表观遗传重编程是不完整的，通常基因组是高度甲基化的。 DNA甲基化抑制剂5-氮杂2'-脱氧胞苷（5-氮杂-dC）可以提高牛，猪，猫和人SCNT胚胎的发育能力。然而，物种间5-氮杂-dC处理的参数不同，并且5-氮杂-dC是否可以增强猪克隆胚胎的发育能力还没有得到很好的研究。因此，在这项研究中，我们对猪胎儿成纤维细胞（PFF）进行了处理，然后将其用作供体核，用5-氮杂-dC以及5-氮杂的浓度和时间依赖性效应将其用作核移植或成纤维细胞衍生的重建胚胎的供体核。通过评估伪造核形成，发育潜力和这些重建胚胎的多能基因表达，研究了猪克隆胚胎上的-dC。我们的结果表明5-氮杂-dC显着降低了PFF中的DNA甲基化水平（0 nM vs. 10 nM vs. 25 nM vs. 50 nM，58.70％vs. 37.37％vs. 45.43％vs. 39.53％，P

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