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首页> 外文期刊>The Open Drug Delivery Journal >A New Oil-Based Antigen Delivery Formulation for both Oral and Parenteral Vaccination
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A New Oil-Based Antigen Delivery Formulation for both Oral and Parenteral Vaccination

机译：用于口服和肠胃外疫苗接种的新型油基抗原递送配方

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The ability of an oil-based carrier vehicle to act as an antigen delivery system via the oral and/or parenteralroutes was investigated. The formulation consists of hydrophilic macromolecules (antigens) solubilised in oil phase, in theabsence of water, by virtue of being wrapped in a sheath of phospholipid amphiphile. Results obtained demonstrate thatthe level of mucosal IgA antibodies detected in the stools of mice immunised orally with cholera toxin B fragment (CTB)or E. coli heat-labile toxin (LT) in oil is much higher than the level of IgA produced by mice immunised with CTB or LTalone. In addition, mice immunised orally with Y. pestis antigens (F1 and V) and CTB as immunostimulant in oil producea significantly increased (p<0.02) systemic IgG response against both antigens (F1 and V) than mice orally immunisedwith F1, V and CTB without oil. Six out of ten mice immunised with F1 and V antigens in oil survived an aerosol challengeof 100 LD50 doses of virulent Y. pestis. Furthermore, animals immunised sub-cutaneously with the HIV antigen(HGP-30) in oil induced much stronger humoral and cellular responses against the antigen than mice immunised with theantigen alone. Taken together, these findings indicate that oil can be used successfully as an antigen delivery system invaccine formulations without the necessity of an aqueous phase or an emulsification process. This greatly enhances thestability and ease of production of a formulation manufactured for commercial use.
机译：研究了油基载体通过口服和/或肠胃外途径作为抗原递送系统的能力。该制剂由亲水性大分子（抗原）组成，该亲水性大分子（抗原）通过包裹在磷脂两亲物的鞘中而在水不存在下溶解。获得的结果表明，口服霍乱毒素B片段（CTB）或大肠杆菌中不耐热毒素（LT）免疫的小鼠粪便中检测到的粘膜IgA抗体水平远高于免疫小鼠产生的IgA水平使用CTB或LTalone。此外，与经F1，V和CTB口服免疫的小鼠相比，口服以鼠疫耶尔森氏菌抗原（F1和V）和CTB作为免疫刺激剂免疫的小鼠产生的针对这两种抗原（F1和V）的全身IgG应答明显增加（p <0.02）。没有油。用油中的F1和V抗原免疫的十分之六的小鼠在100 LD50剂量的鼠疫耶尔森氏菌的气溶胶攻击中幸存下来。此外，与仅用抗原免疫的小鼠相比，用油中的HIV抗原（HGP-30）进行皮下免疫的动物对抗原的体液和细胞反应强烈得多。综上所述，这些发现表明，油可以成功地用作疫苗制剂的抗原递送系统，而无需水相或乳化过程。这极大地增强了为商业用途制造的制剂的稳定性和易于生产性。

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