Two different approaches to the creation of “rigid” (gel-like) DNA particles (or DNA nanoconstructions) areconsidered. The physicochemical approach is based on the formation of nanobridges between double-stranded DNAmolecules ordered in quasinematic layers of cholesteric liquid-crystalline dispersion (CLCD) particles or their salting-out.This approach represents by itself a “chemical gelation” of DNA molecules realized inside the nanometric DNA particles.The formed “rigid” DNA particles have unique physicochemical properties. The nanotechnological approach is based oninduction of “physical gelation” as a result of the formation of Au-clusters in the “free” space between double-strandedDNA molecules ordered in CLCD particles. This approach results in the formation of a new “rigid” DNA material. Its distinctivepeculiarity, in contrast to the chemically bonded “rigid” DNA particles, consists in a very weak binding affinity tothe nuclear membrane filter used for atomic force microscopy. This opens up a possibility for easy manipulations withCLCD particles doped with gold nanoparticles.
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