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首页> 外文期刊>The Open Inflammation Journal >Effects of Repeated Antigen Exposure on the Expression of Enzymes Associated with Sphingosine-1-Phosphate Turnover
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Effects of Repeated Antigen Exposure on the Expression of Enzymes Associated with Sphingosine-1-Phosphate Turnover

机译：反复暴露于抗原对鞘氨醇-1-磷酸转换相关酶表达的影响

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摘要

Increasing evidence suggests that sphingosine-1-phosphate (S1P) is involved in the pathogenesis of allergic bronchial asthma. In the present study, the changes in the expression levels of enzymes associated with S1P turnover were determined in a mouse model of allergic bronchial asthma. Male BALB/c mice were actively sensitized with ovalbumin antigen, and were repeatedly challenged with aerosolized antigen. Twenty-four hours after the last antigen, total RNAs were extracted from lungs. The RT-PCR analyses revealed that mRNAs for the S1P-producing enzymes, sphingosine kinase 1 (SphK1) and SphK2, and for the S1P-breakdown enzymes, S1P phosphatase 1 (S1PP1), S1PP2, S1P lyase, and lipid phosphate phosphatase 1a, were expressed in the lungs of mice. Among them, the mRNA levels of SphK2 and S1PP1 were significantly increased in the lungs of the repeatedly antigen-challenged mice. It is thus possible that the S1P turnover is increased in the airways of allergic bronchial asthma.
机译：越来越多的证据表明1-磷酸鞘氨醇（S1P）参与了过敏性支气管哮喘的发病机理。在本研究中，在过敏性支气管哮喘的小鼠模型中确定了与S1P周转相关的酶表达水平的变化。雄性BALB / c小鼠被卵白蛋白抗原主动致敏，并被雾化抗原反复攻击。最后一种抗原后二十四小时，从肺中提取总RNA。 RT-PCR分析显示，产生S1P的酶鞘氨醇激酶1（SphK1）和SphK2的mRNA，以及针对S1P分解酶的S1P磷酸酶1（S1PP1），S1PP2，S1P裂合酶和脂质磷酸化磷酸酶1a的mRNA，在小鼠的肺中表达。其中，反复受到抗原攻击的小鼠的肺中SphK2和S1PP1的mRNA水平显着升高。因此，过敏性支气管哮喘的气道中S1P转换可能增加。

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