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首页> 外文期刊>Tropical Journal of Pharmaceutical Research >Isolation and identification of flavonoids from anticancer and neuroprotective extracts of Trigonella foenum graecum
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Isolation and identification of flavonoids from anticancer and neuroprotective extracts of Trigonella foenum graecum

机译：胡芦巴抗癌及神经保护性提取物中黄酮类化合物的分离与鉴定

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Purpose: To evaluate the protective effects of Trigonella foenum graecum methanol and ethyl acetate extracts, against cancer cell lines and NaNO 2 -induced neurodegeneration in mice brain. Methods: Adult male albino mice (n = 20) were administered NaNO 2 orally at a dose of 300 mg/kg for 15 days. The control group received distilled water and normal mice feed. Experimental groups were given T. foenum graecum methanol and ethyl acetate extracts in two different doses of 100 and 200 mg/kg orally for 15 days. Histopathological examination of the brain was carried out with the aid of cresyl violet and H&E staining. In addition, the cytotoxicity of the extracts was evaluated by 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay against HCEC, MCF-7 and Hep2 cell lines. Nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS) were used to determine the structures of the bioactive compounds. Results: Methanol and ethyl acetate extracts of T. foenum graecum seeds inhibited neurodegeneration in the hippocampus and cortex regions of the brain when compared to control group. Moreover, the extracts exhibited anticancer activity against Hep2 and MCF-7cells and low cytotoxicity against HCEC, sparing healthy cells in-vitro. In addition, two flavonoids amurensin and cosmosiin were isolated from T. foenum graecum extracts. Conclusion: Amurensin and cosmosiin from T. foenum extracts are reported here for the first time agents that possess significant anticancer and neuroprotective properties.
机译：目的：评价粉状Trigonella foenum graecum甲醇和乙酸乙酯提取物对小鼠脑癌细胞系和NaNO 2诱导的神经变性的保护作用。方法：成年雄性白化病小鼠（n = 20）以300 mg / kg的剂量口服NaNO 2 15天。对照组接受蒸馏水和正常小鼠饲料。实验组分别以100和200 mg / kg的两种不同剂量口服给予风叶草甲醇和乙酸乙酯提取物15天。借助甲酚紫和H＆E染色对大脑进行组织病理学检查。此外，通过3-（4,5-二甲基噻唑基）-2,5-二苯基溴化四溴甲烷（MTT）分析评估提取物对HCEC，MCF-7和Hep2细胞系的细胞毒性。核磁共振（NMR）和电喷雾电离质谱（ESI-MS）用于确定生物活性化合物的结构。结果：与对照组相比，黑麦草种子的甲醇和乙酸乙酯提取物抑制了大脑海马和皮质区域的神经变性。此外，提取物表现出对Hep2和MCF-7细胞的抗癌活性，并且对HCEC的细胞毒性低，从而在体外不影响健康细胞。此外，还从禾本科提取物中提取了两种类黄酮amurensin和cosmosiin。结论：本文首次报道了来自茴香提取物的Amurensin和cosmosiin具有显着的抗癌和神经保护特性。

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