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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Molecular identification of bovine papillomaviruses in dairy and beef cattle: first description of Xi- and Epsilonpapillomavirus in Turkey
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Molecular identification of bovine papillomaviruses in dairy and beef cattle: first description of Xi- and Epsilonpapillomavirus in Turkey

机译：奶牛和肉牛中牛乳头瘤病毒的分子鉴定：土耳其的Xi和Epsilonpapillomavirus 的首次描述

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In the current study, 23 papilloma/tumor-like samples obtained from cattle having clinical lesions and 9 blood samples collected from healthy-appearing cattle in Turkey were examined for bovine papillomavirus (BPV) DNA using the degenerate primers FAP59/64, and the different types of BPV were distinguished by type-specific primer sets. Furthermore, histopathological studies of papillomavirus were performed. A total of 7 BPV types (BPVs 1, 2, 3, 4, 6, 8, 9), including genera Deltapapillomavirus, Xipapillomavirus, and Epsilonpapillomavirus , were identified. In all samples, BPV-1 was the most common genotype (90.6%). Overall, coinfections were determined in 26 of the examined samples (81.3%), and coinfection with BPV-1/BPV-4/BPV-8 (21.9%) was the most frequently identified using BPV type-specific primers. Moreover, bovine leukemia virus, an oncogenic retrovirus, was detected from three cattle with tumor-like lesions (13.0%), which were also coinfected by different BPV types. Histopathologically, nine papilloma-like lesions were assessed and diagnosed as five fibropapillomas and four papillomas. BPV infection is an important cause of economic losses in the dairy and beef industry. Our study will be highly useful for guiding further large-scale epidemiological studies and providing detailed data on the risk factors associated with BPV infection in cattle populations in Turkey.
机译：在本研究中，使用简并引物FAP59 / 64检查了23例从具有临床病变的牛中获得的乳头状瘤/肿瘤样样品和9例从健康的牛身上采集的9份血样中牛乳头瘤病毒（BPV）DNA， BPV的类型通过类型特异性引物集来区分。此外，进行了乳头瘤病毒的组织病理学研究。共鉴定出7种BPV类型（BPV 1、2、3、4、6、8、9），包括Deltapapillomavirus病毒，Xipapillomavirus病毒和Epsilonpapillomavirus病毒。在所有样品中，BPV-1是最常见的基因型（90.6％）。总体而言，在26个被检样品中确定了共感染（81.3％），而BPV-1 / BPV-4 / BPV-8（21.9％）的共感染是使用BPV类型特异性引物确定的最高频率。此外，从三头有肿瘤样病变（13.0％）的牛中检出了牛白血病病毒（一种致癌逆转录病毒），这些牛也被不同的BPV类型共同感染。在组织病理学上，评估并诊断出9个乳头状瘤样病变，诊断为5个纤维乳头状瘤和4个乳头状瘤。 BPV感染是乳制品和牛肉行业经济损失的重要原因。我们的研究对于指导进一步的大规模流行病学研究以及提供与土耳其牛群中与BPV感染相关的危险因素的详细数据非常有用。

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