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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Comparative evaluation of factors affecting hemagglutinatingactivity of avian influenza (H9) virus
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Comparative evaluation of factors affecting hemagglutinatingactivity of avian influenza (H9) virus

机译：禽流感（H9）病毒血凝活性影响因素的比较评估

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摘要

Influenza virus H9 was standardized for hemagglutination assay using different factors such as red blood cell (RBC) types, concentrations, diluent types, and storage times. Avian influenza virus H9 was grown in embryonated chicken eggs and confirmed by spot agglutination. A significant (P 0.05) were found between human blood type O, chicken, and dog RBCs, as well as among rabbit, pigeon, sheep, and parrot. The highest titer (9.00 ± 0.00) with a nonsignificant difference was found using virus stored at 4 °C and -20 °C while 37 °C showed the lowest significant mean hemagglutinin (HA) titer (11.08 ± 188.21). Nonsignificant differences were observed in HA titers against H9 virus stored for 3, 4, 5, and 6 months. Nonsignificant differences were found between the use of normal saline and 0.5% peptone water with the lowest HA titers of 7.83 ± 0.40 and 8.00 ± 0.00, respectively, while the highest HA titer (9.00 ± 0.00) with nonsignificant difference was observed by using HA-HI buffer and phosphate buffer saline as diluents. RBCs with 0.5% and 1% concentrations showed nonsignificant difference in HA titer but significant difference with 0.1% RBCs.
机译：使用不同因素（例如红细胞（RBC）类型，浓度，稀释剂类型和保存时间）将流感病毒H9标准化用于血凝测定。禽流感病毒H9在带有胚的鸡蛋中生长，并通过点凝集确认。在人血型O，鸡和狗的RBC之间以及兔子，鸽子，绵羊和鹦鹉之间发现了显着性（P 0.05）。使用在4°C和-20°C下储存的病毒，发现最高滴度（9.00±0.00）没有显着差异，而37°C则显示最低的平均血凝素（HA）滴度（11.08±188.21）。在针对H9病毒保存的3、4、5和6个月的HA滴度中未观察到显着差异。在使用生理盐水和0.5％蛋白ept水之间发现无显着差异，HA滴度最低，分别为7.83±0.40和8.00±0.00，而使用HA-观察到最高HA滴度（9.00±0.00），无显着差异。 HI缓冲液和磷酸盐缓冲液作为稀释剂。浓度为0.5％和1％的RBC的HA滴度无显着差异，而0.1％的RBC则具有显着差异。

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