首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Molecular characterization of the bovine noroviruses from diarrheic calves in Turkey
【24h】

Molecular characterization of the bovine noroviruses from diarrheic calves in Turkey

机译:土耳其腹泻牛的诺如病毒的分子特征

获取原文
           

摘要

In this study, we performed a survey to detect bovine noroviruses (BNoVs) in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by nested reverse-transcription RT-PCR, using primers located in the consensus sequences of the BNoV RNA-dependent RNA polymerase ( RdRp ) gene. The BNoV RdRp gene was detected in 1.7% (4/235) of samples by nested RT-PCR. The nucleotide sequences of partial RdRp fragments from the BNoV isolates including the newly identified Turkish isolates showed more than 86.3% nt and 96.8% aa identity with each other. Interestingly, in a comparison of the nt and aa sequences, one of the Turkish BNoV strains was found to be 100% aa identical with some Italian and Tunisian strains. In conclusion, BNoV is one of the pathogens that contributes to neonatal calf diarrhea cases in Turkey.
机译:在这项研究中,我们进行了一项调查,以检测2009年至2011年间土耳其的牛诺如病毒(BNoV),方法是使用235例新生儿腹泻的粪便样本,通过嵌套逆转录RT-PCR分析这些样本,方法是使用位于BNoV RNA依赖的RNA聚合酶(RdRp)基因。通过巢式RT-PCR在1.7%(4/235)的样品中检测到BNoV RdRp基因。来自BNoV分离株的部分RdRp片段的核苷酸序列(包括新近鉴定的土耳其分离株)显示出超过86.3%nt和96.8%aa的同一性。有趣的是,在比较nt和aa序列时,发现土耳其的一种BNoV菌株与某些意大利和突尼斯的菌株具有100%的aa一致性。总之,BNoV是导致土耳其小牛腹泻的一种病原体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号