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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Effect of capacitating agents on sperm pretreatment during in vitro fertilization for blastocyst production in caprines
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Effect of capacitating agents on sperm pretreatment during in vitro fertilization for blastocyst production in caprines

机译：电容剂对体外受精过程中精子预处理的影响

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摘要

The present study aimed at comparing the relative efficacy of different capacitating agents used for sperm pretreatment during in vitro fertilization (IVF) and subsequent embryo development of caprine oocytes. In experiment 1, the effect of different concentrations of heparin (0, 20, and 50 μg/mL) on sperm pretreatment for different periods of time (30 and 60 min) was studied. In experiment 2, the dose-dependent effect of calcium ionophore (0, 0.1, 0.2, and 0.5 μM) on sperm pretreatment was assessed. Experiment 3 validated and compared the effects of both capacitating agents on sperm pretreatment for capacitation based on the results of the first two experiments. The treated sperms from each group were used for in vitro fertilization and subsequent embryo development. The results indicated that in experiment 1, capacitation with heparin at 20 μg/mL for 60 min had significantly higher (P
机译：本研究旨在比较用于体外受精（IVF）和随后的山羊卵母细胞胚胎发育过程中精子预处理的不同电容剂的相对​​功效。在实验1中，研究了不同浓度的肝素（0、20和50μg/ mL）在不同时间段（30和60分钟）对精子预处理的影响。在实验2中，评估了钙离子载体（0、0.1、0.2和0.5μM）对精子预处理的剂量依赖性作用。实验3基于前两个实验的结果，验证并比较了两种电容剂对精子预处理的影响。每组处理过的精子用于体外受精和随后的胚胎发育。结果表明，在实验1中，以20μg/ mL的肝素进行60分钟的获能明显更高（P

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