• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Comparative evaluation of sequence analysis of 16S rRNA and rpoB genes for identification of aquatic mycobacteria
【24h】

Comparative evaluation of sequence analysis of 16S rRNA and rpoB genes for identification of aquatic mycobacteria

机译：16S rRNA和 rpoB 基因序列分析用于鉴定水生分枝杆菌的比较评价

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The nucleotide sequences of partial 16S rRNA and bacterial RNA polymerase ?-subunit ( rpoB ) genes for 57 mycobacterial strains were determined. Compared to the 16S rRNA gene sequences, variable regions were scattered along the whole fragment sequence, indicating that the rpoB gene is more polymorphic. Unlike 16S rRNA sequences, species variation was observed within M. fortuitum strains. The topology of the rpoB -based phylogenetic tree was almost the same as that of the 16S rRNA sequence analysis. These results suggest that the rpoB gene is a highly conserved gene, and taxonomical studies based on this gene may be comparable with similar studies based on the 16S rRNA gene. The overall mean distance for rpoB -gene?based sequences was 2.5 times greater than that of the 16S rRNA gene for all 57 mycobacterial strains examined. However, some slowly growing mycobacteria could not be differentiated based on rpoB gene sequences. Moreover, a bootstrap value above 70% was observed for 13 nodes, while this value was 14 nodes in the case of 16S rRNA sequences. To the best of our knowledge, this is the first investigation evaluating the use of 16S rRNA and rpoB sequence analyses for identification of aquatic mycobacteria obtained from diverse geographical locations.
机译：确定了57个分枝杆菌菌株的部分16S rRNA和细菌RNA聚合酶β-亚基（rpoB）基因的核苷酸序列。与16S rRNA基因序列相比，可变区沿整个片段序列分散，表明rpoB基因更具多态性。与16S rRNA序列不同，在M. fortuitum菌株中观察到物种变异。基于rpoB的系统发育树的拓扑与16S rRNA序列分析的拓扑几乎相同。这些结果表明，rpoB基因是高度保守的基因，基于该基因的分类学研究可与基于16S rRNA基因的类似研究相媲美。对于所有57个分枝杆菌菌株，基于rpoB基因的序列的总平均距离比16S rRNA基因的总平均距离大2.5倍。但是，根据rpoB基因序列无法区分一些缓慢生长的分枝杆菌。此外，对于13个节点，观察到高于70％的引导值，而对于16S rRNA序列，该值为14个节点。据我们所知，这是首次评估使用16S rRNA和rpoB序列分析鉴定从不同地理位置获得的水生分枝杆菌的研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号