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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Lactose-rich milk replacer modifies the proteome of bloodplasma in 2-week-old calves
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Lactose-rich milk replacer modifies the proteome of bloodplasma in 2-week-old calves

机译：富含乳糖的牛奶代用品可修饰2周龄小牛的血浆蛋白质组

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The aim of the study was to create a specific pattern of protein expression changes in the plasma of 2-week-old calves treated with excessive lactose doses. Identification of proteins, the expression of which changes during the short-term administration of lactose, may help to exclude or confirm the causes of diarrhea observed in newborn calves. The present study utilized 2-D electrophoresis combined with MALDI-TOF mass spectrometry and identified 6 blood plasma proteins, the expression of which changed under the influence of lactose added to the milk replacer. The pattern of expression of blood plasma proteins, including decreased expression of fibrinogen and apolipoprotein A-IV and increased expression of alpha-1B-glycoprotein, alpha-1-antiproteinase, and apolipoprotein E in 2-week-old calves fed with lactose-rich milk replacer, can be used to determine the causes of diarrhea in calves.
机译：该研究的目的是在用过量乳糖治疗的2周龄小牛的血浆中创建一种特定的蛋白质表达变化模式。鉴定蛋白质（其表达在短期给予乳糖期间会发生变化）可能有助于排除或确认新生牛犊腹泻的原因。本研究利用二维电泳与MALDI-TOF质谱联用，鉴定了6种血浆蛋白，其表达在添加到牛奶代用品中的乳糖的影响下发生了变化。富含乳糖的2周龄犊牛血浆蛋白表达模式包括纤维蛋白原和载脂蛋白A-IV的降低以及α-1B-糖蛋白，α-1-抗蛋白酶和载脂蛋白E的表达增加牛奶替代品，可用于确定小牛腹泻的原因。

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