• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >The combination of CASA kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen
【24h】

The combination of CASA kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen

机译：CASA动力学参数和荧光素染色的组合作为低温保存牛精液的育性工具

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Prediction of male fertility with in vitro semen quality parameters remains a challenge for the bull industry. Fluorescein staining and computer-assisted semen analysis (CASA) ensure kinetically proper and functionally objective results to improve spermatological parameter control. Therefore, we aimed to examine the CASA kinetic parameters and fluorescein staining of cryopreserved bull semen and its relationship with fertility. A total of 90 frozen straws from 5 Simmental bulls were evaluated. After semen was thawed, the motility, kinetic parameters, and insemination doses were evaluated by CASA; fluorescein stainings were evaluated using a fluorescein microscope. The 60th-day nonreturn rates (NRRs) were recorded for determination of pregnancy rates. There were significant differences in motility and kinetic parameters among bulls. Bull 3 had the lowest percentage of NRR and curvilinear velocity, straight line velocity, beat-cross frequency, and lateral head displacement parameters (P 0.05). A positive correlation was detected between the pregnancy rate and some kinetic parameters. The correlation between the CASA parameters was determined as well. We concluded that various kinetic parameters obtained with CASA software system algorithms and fluorescein stainings can be linked to fertility. However, further research is needed to acquire a more precise link with fertility.
机译：用体外精液质量参数预测雄性育性仍然是牛市的挑战。荧光素染色和计算机辅助精液分析（CASA）确保在动力学上和功能上客观的结果，以改善精子学参数控制。因此，我们旨在研究冷冻保存的公牛精液的CASA动力学参数和荧光素染色及其与生育力的关系。评估了来自5只西门塔尔牛的90根冷冻稻草。解冻精液后，通过CASA评估其运动能力，动力学参数和授精剂量。使用荧光显微镜观察荧光素染色。记录第60天的非返回率（NRR），以确定怀孕率。在公牛之间，运动性和动力学参数存在显着差异。公牛3的NRR和曲线速度，直线速度，心跳频率和横向头部位移参数的百分比最低（P <0.05）。在妊娠率和一些动力学参数之间检测到正相关。还确定了CASA参数之间的相关性。我们得出的结论是，使用CASA软件系统算法和荧光素染色获得的各种动力学参数可以与生育力相关。但是，需要进一步的研究来获得与生育率更精确的联系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号