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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Isolation of iArcobacter/i spp. from domestic ducks and geese and identification of the recovered isolates by using molecular method
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Isolation of iArcobacter/i spp. from domestic ducks and geese and identification of the recovered isolates by using molecular method

机译：Arcobacter spp的分离。家禽和鹅的分离和分子分离方法鉴定

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This study aimed to investigate the role of healthy domestic geese and ducks as Arcobacter carriers. A total of 599 samples, including 330 cloacal swab and 116 fecal samples from geese, and 153 cloacal swab samples from ducks raised on family farms were analysed. For this purpose, the membrane filtration method was applied. In this study, Arcobacter spp. were isolated at rates of 16.7% and 12.93% from cloacal swab and stool samples of geese, respectively, and 26.14% from cloacal swab samples of ducks. Obtained isolates were identified by multiplex PCR (m-PCR) as A. cryaerophilus, A. butzleri , and A. skirrowii . Seasonal analyses of Arcobacter spp. that were isolated from the examined samples were done for the months in which the samples were collected. While the highest rate of Arcobacter spp. in cloacal swab samples in geese was obtained in October, Arcobacter spp. was found in stool samples in July. The highest isolation rate for ducks was obtained in July as well. These isolation rates suggest that the stool may play an important role in the transmission and spread of arcobacters. Consequently, ducks and geese, which are reservoirs for arcobacters because they carry Arcobacter spp. in their digestive systems, play a considerable role in the transmission of arcobacters to other animals and to humans, thereby being vectors of infection.
机译：这项研究旨在调查健康的家养鹅和鸭作为弓形杆菌载体的作用。共分析了599份样品，包括330份泄殖腔拭子和116只鹅粪便样本，以及153份泄殖腔拭子，这些样本来自家庭农场饲养的鸭子。为此，采用了膜过滤法。在这项研究中，Arcobacter spp。分别从鹅的泄殖腔拭子和粪便样本中分离出16.7％和12.93％的比率，从鸭子的泄殖腔拭子样本中分离出26.14％的比率。通过多重PCR（m-PCR）将获得的分离物鉴定为嗜冷链球菌，butzleri菌和skirrowii菌。弓形杆菌的季节性分析。从检查的样品中分离出来的样品在收集样品的月份中进行。而弓形杆菌属菌种率最高。鹅的泄殖腔拭子样品是在十月份的Arcobacter spp中获得的。在七月的粪便样本中被发现。七月份，鸭的隔离率也最高。这些隔离率表明粪便可能在弧菌的传播和传播中起重要作用。因此，鸭和鹅是弓形细菌的储存库，因为它们携带了杆状杆菌属。在其消化系统中，圆弧菌在向其他动物和人类的传播中起着相当重要的作用，因此是感染的载体。

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