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Comparison of different paternity test panels in sheep

机译:绵羊不同亲子鉴定板的比较

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The aims of this study were to evaluate different paternity test panels with 17 microsatellite markers for their effectiveness in paternity analysis and to identify pedigree error rates in the Kangal Akkaraman breed of sheep. The animal material for the study consisted of 175 Kangal Akkaraman sheep, 35 rams, and their 140 offspring, raised on farms and bred using controlled mating. The panels were created according to probability of exclusion (PE) of 17 studied microsatellites. A total of 240 alleles were detected across 17 microsatellite loci. The overall mean value of the polymorphic information content (PIC) (0.78) indicated that the microsatellite panels were highly polymorphic. Probability of identity (PI) values ranged between 0.02 and 0.13. It can be said that pedigree error (2.94%) occurred at a low rate in this study. The highest combined PE values were obtained from Panel-16 (0.9999771) as expected. Panel-8 and Panel-9 met the PE value accepted in the literature (0.999). The results show that these panels are cheaper and more practical than other panels formed for Kangal Akkaraman.
机译:这项研究的目的是评估具有17个微卫星标记的不同亲子鉴定小组在亲子鉴定中的有效性,并确定Kangal Akkaraman绵羊品种的系谱错误率。用于研究的动物材料包括175只袋鼠Akkaraman绵羊,35只公羊及其140个后代,它们是在农场饲养并通过控制交配繁殖的。根据17个研究的微卫星的排除概率(PE)创建面板。在17个微卫星基因座上共检测到240个等位基因。多态信息含量(PIC)的整体平均值(0.78)表明微卫星面板是高度多态的。同一性(PI)值的范围在0.02和0.13之间。可以说,本研究中的系谱错误发生率低(2.94%)。从面板16(0.9999771)获得了最高的预期PE值。 Panel-8和Panel-9达到了文献(0.999)接受的PE值。结果表明,与为Kangal Akkaraman形成的其他面板相比,这些面板更便宜,更实用。

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