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Effects of organic and inorganic nitrogen sources on in vitro degradability of citrus byproduct and milk thistle

机译:有机和无机氮源对柑桔副产物和乳蓟体外降解的影响

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In order to evaluate rumen microbial activity, the effects of two nitrogen sources (organic and inorganic) were analyzed in vitro by inoculating dairy cow rumen fluid and using citrus byproduct (CBP) and milk thistle ( Silybum marianum , MT) as energy source substrates. The experimental design was 2 × 3 where we used three different nitrogen sources (N1: no nitrogen added, N: ammonium bicarbonate, N3: sodium glutamate) with a basic concentration of nitrogen (100 mg/1 L). Culture pH, dry matter degradability %, ammonia-N (NH$_{3}$-N), total volatile fatty acids (TVFAs), individual volatile fatty acid proportions, and acetate propionate ratio (A:P) were measured after 24 h of fermentation. Culture pH values were in the optimal range with CBP and MT in all media. Supplementing nitrogen sources had similar tendencies to increase dry matter degradability (P.
机译:为了评估瘤胃微生物活性,通过接种奶牛瘤胃液并使用柑橘副产物(CBP)和水飞蓟(Silybum marianum,MT)作为能源底物,体外分析了两种氮源(有机和无机)的作用。实验设计为2×3,其中我们使用了三种基本氮浓度为100 mg / 1 L的氮源(N1:未添加氮,N:碳酸氢铵,N3:谷氨酸钠)。在24小时后,测量培养液的pH,干物质降解率%,氨氮(NH $ _ {3} $-N),总挥发性脂肪酸(TVFA),各个挥发性脂肪酸的比例和乙酸丙酸酯比(A:P)。发酵小时。在所有培养基中,使用CBP和MT的培养液pH值均在最佳范围内。补充氮源具有增加干物质降解能力的相似趋势(P.

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