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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand
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Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand

机译：泰国北部与牛乳腺炎相关的乳房链球菌的毒力相关基因和分子分型

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Streptococcus uberis is a major bovine mastitis pathogen. Several studies have revealed a persistence of specific strains of S. uberis with enhanced virulence or transmissibility. We aimed to investigate the presence of S. uberis virulence-associated genes including plasminogen activator (pauA) , glyceraldehyde-3-phosphate dehydrogenase (gapC) , oligopeptide permease (oppF) , metal transporter uberis A (mtuA) , hyaluronic acid capsules (hasA, hasB, hasC) , lactoferrin binding protein (lbp) , adhesion protein (sua) , and CAMP factor (cfu) as well as the genetic profiles using pulsed-field gel electrophoresis (PFGE) among isolates from cases of clinical mastitis, subclinical mastitis, and intramammary infection with low somatic cell count in dairy cows. A total of 642 milk samples from 178 milking cows of 53 farms in Chiang Mai, Thailand, were collected and cultured. Eighty-eight S. uberis isolates were identified. The most common pattern of virulence-associated genes was hasA+hasB+hasC+sua+gapC+lbp+pauA+oppF+mtuA (34.1%, 30/88). PFGE was performed with 71 isolates, which could be classified into 56 pulsotypes. S. uberis isolates tended to be clustered by farms regardless of the severity of infection. Therefore, molecular epidemiological investigation can be useful in developing an effective control strategy for bovine mastitis caused by S. uberis in dairy farms.
机译：乳房链球菌是主要的牛乳腺炎病原体。几项研究表明，持久存在的乳房链球菌特定菌株具有更高的毒力或可传播性。我们旨在调查乳房链球菌毒力相关基因的存在，包括纤溶酶原激活物（pauA），甘油醛-3-磷酸脱氢酶（gapC），寡肽通透酶（oppF），金属转运体乳房risA（mtuA），透明质酸胶囊（hasA ，hasB，hasC），乳铁蛋白结合蛋白（lbp），粘附蛋白（sua）和CAMP因子（cfu）以及使用脉冲场凝胶电泳（PFGE）分离的临床乳腺炎，亚临床乳腺炎病例的遗传特征以及奶牛体细胞计数低的乳内感染。收集并培养了来自泰国清迈53个农场的178头奶牛的642份牛奶样品。鉴定出88个乳房链球菌。与毒力相关的基因最常见的模式是hasA + hasB + hasC + sua + gapC + lbp + pauA + oppF + mtuA（34.1％，30/88）。 PFGE用71个分离株进行，可分为56个脉冲型。无论感染的严重程度如何，乳房链球菌的分离株都倾向于被农场聚集。因此，分子流行病学调查对于开发由奶牛场中乳房链球菌引起的牛乳腺炎的有效控制策略可能是有用的。

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