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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Co-existence of bovine viral diarrhea and border disease viruses in a sheep flock suffering from abortus and diarrhea
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Co-existence of bovine viral diarrhea and border disease viruses in a sheep flock suffering from abortus and diarrhea

机译：牛病毒性腹泻和边界疾病病毒在流产和腹泻的羊群中并存

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Pestivirus infections have a huge economic impact on livestock production. In 2014, an aborted fetus from a sheep flock suffering from abortus and diarrhea was submitted for virological diagnosis. Due to the positive result of the sample for pestivirus and continuing clinical symptoms in the flock, all of the animals were sampled individually. Blood samples for serum and peripheral blood mononuclear cells were collected from 93 animals (5 rams, 26 lambs, and 62 sheep). During sampling 1 ocular and 4 rectal swab samples were obtained from lambs that had a clinical eye problem and diarrhea, respectively. Additionally 5 aborted fetuses were submitted after the initial sampling. Thirteen of the 93 blood samples tested positive for pestivirus by antigen-detection ELISA. Propagation of noncytopathogenic virus was detected in blood samples from 6 lambs and in 1 aborted fetus sample by using the indirect immunoperoxidase monolayer assay. Pestivirus RNA was detected in 10 of 13 samples by RT-PCR employing pan-pestivirus primers. Border disease virus (BDV) RNA was identified with PBD1/PBD2 specific primers in all 10 samples that tested positive for pan-pestivirus primers. Differentiating RT-PCR further identified BVDV-1 sequences in 3 of the 10 samples. The Sequenced BDV strain (KY-57) was located in the cluster of BDV-7 (Aydin-like) while the BVDV strain was close close to BVDV-1c. The results of this study highlight the possibility of dual infection in sheep with BDV and BVDV-1.
机译：瘟病毒感染对畜牧业产生巨大的经济影响。 2014年，患有流产和腹泻的羊群流产胎儿提交了病毒学诊断。由于瘟病毒样品的阳性结果和羊群中持续的临床症状，所有动物均单独取样。从93只动物（5只公羊，26只羊羔和62只绵羊）中收集了血清和外周血单核细胞的血样。在采样过程中，分别从患有临床眼病和腹泻的羊羔身上采集了1眼和4支直肠拭子样品。初始采样后，另外提交了5名流产胎儿。 93份血液样本中有13份通过抗原检测ELISA检测为瘟病毒呈阳性。通过使用间接免疫过氧化物酶单层测定，在6只羔羊的血液样本和1个流产的胎儿样本中检测到非致病性病毒的传播。使用泛瘟病毒引物通过RT-PCR在13个样品中的10个中检测到瘟病毒RNA。在所有10份泛瘟病毒引物检测呈阳性的样品中，都使用PBD1 / PBD2特异性引物鉴定了边界病病毒（BDV）RNA。区分RT-PCR进一步鉴定了10个样品中的3个中的BVDV-1序列。测序的BDV株（KY-57）位于BDV-7（Aydin-like）的群集中，而BVDV株则接近BVDV-1c。这项研究的结果凸显了BDV和BVDV-1在绵羊中双重感染的可能性。

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