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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Intrauterine insemination with cat semen frozen with various extenders
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Intrauterine insemination with cat semen frozen with various extenders

机译：用各种补充剂冷冻的猫精液进行宫内授精

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The aim of this study was to investigate the post-thaw motility and morphological defect rates of cat semen frozen with various extenders, and pregnancy rates after intrauterine insemination (IUI). Cats, 2 males and 14 queens, 2 to 3 years of age, and under the same managemental conditions were used. Semen was collected by means of an electro-ejaculator under general anesthesia. Based on spermatological traits, semen samples presumed suitable for freezing were extended with either tris-fructose-citric acid (EY-TFC) containing 20% egg yolk, or skimmed milk-glucose-taurine (EY-SMGT) containing 10% egg yolk extender, and frozen in 0.25 mL straws. For the extenders, post-thaw motility and morphological abnormality values were found to be efficient for IUI and no statistical significance was observed between the values of the extender groups. To achieve ovulation, queens showing behavioral estrus received 250 IU of hCG via IM route, 30 h prior to IUI. Under general anesthesia, queens were laparatomised on the medial line and the left and right uterine horn were taken out. Into both uterine horns, 2 straws containing 25 × 106 sperm each were thawed at 37 °C for 30 s and equally injected. Pregnancy was diagnosed using an ultrasonography device on the 19th day after IUI by detecting the embryonic vesicles. Of the 3 EY-TFC group queens found to be pregnant, 1 aborted and other 2 had early embryonic death (EED); the pregnancy rate was 42.90%. In a queen in the EY-SMGT group, no ovulation took place after the hCG injection. In 2 of the queens found to be pregnant in this group, EED occurred and the pregnancy rate was 33.30%. In conclusion, cat semen extended and frozen with EY-TFC and EY-SMGT extenders was found to be successful in post-thaw motility and morphological defect rates. Therefore, surgical IUI may be more effective for feline AI using frozen semen rather than intravaginal insemination.
机译：这项研究的目的是调查用各种填充剂冷冻的猫精液的解冻后运动性和形态缺陷率，以及宫内授精（IUI）后的妊娠率。使用2至3岁的猫（2头雄性和14头皇后），并在相同的管理条件下使用。在全身麻醉下通过电射精收集精液。根据精子学特征，将假定适合冷冻的精液样品用含有20％蛋黄的三果糖柠檬酸（EY-TFC）或含有10％蛋黄增量剂的脱脂牛奶葡萄糖牛磺酸（EY-SMGT）进行扩展，并用0.25 mL吸管冷冻。对于补充剂，发现解冻后的运动力和形态异常值对于IUI有效，并且在补充剂组的值之间未观察到统计学显着性。为了排卵，表现出行为发情的皇后在IUI前30小时通过IM途径接受了250 IU的hCG。在全身麻醉的情况下，将皇后切入内侧线，取出左子宫角和右子宫角。在37°C融化2根含25×106精子的吸管，分别插入两个子宫角30 s，并平均注入。 IUI后第19天，通过检测胚胎囊泡，使用超声检查仪诊断出妊娠。在发现的3名EY-TFC女王中，有1位流产，另外2位有早期胚胎死亡（EED）;妊娠率为42.90％。在EY-SMGT组的女王中，hCG注射后未发生排卵。该组中有2名皇后怀孕，发生EED，妊娠率为33.30％。总之，发现用EY-TFC和EY-SMGT补充剂延长并冷冻的猫精液在解冻后运动性和形态缺陷率方面是成功的。因此，外科IUI可能对冷冻精液而非阴道内授精的猫AI更有效。

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