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首页> 外文期刊>Veterinarski glasnik >Examining the presence of specific antibodies against Salmonella enteritidis in vaccinated and unvaccinated poultry
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Examining the presence of specific antibodies against Salmonella enteritidis in vaccinated and unvaccinated poultry

机译：检查接种和未接种禽肉中针对肠炎沙门氏菌的特异性抗体的存在

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Introduction. The objective of our research was to point to the significance of serological diagnostic methods, that is, competitive immunoenzyme test - cELISA as well as the method of classical agglutination of O and H salmonella antigen, for specific antibodies against Salmonella enterica subspecies enterica serovar Enteritidis presence and titre detection in blood serum samples of bothvaccinated and unvaccinated poultry. Material and methods. In our work, we have used commercial competitive immunoenzyme test - cELISA and classical agglutination method with O and H antigens of Salmonella enterica subspecies enterica serovar Enteritidis. Comparative testing included 177 blood serum samples of poultry, out of which 137 was from unvaccinated and 40 from vaccinated individuals originating from majority of poutry farms. Results. In 74 blood serum samples, that is 54,01% out of the total of tested samples originating from unvaccinated flocks, by the use of cELISA test, there were found specific antibodies against S. Enteritidis, while by the method of classical agglutination specific antibodies against O antigen 1,9 and 12 were found in 58 samples, that is in 42,34% , and specific antibodies against H antigen g and m were found in 61 samples,what was 44,53% . In all the tested blood serum samples of vaccinated poultry, there was determined the presence of of specific antibodies against S. Enteritidis, both by the use of competitive cELISA method and classical agglutination with somatic O and flagellar H antigens. By the statistical analysis of the results obtained by the method of classical agglutination and cELISA use of kappa test, there was found out a very good compliance (kappa=0,813). Conclusion. Based on the compared results of blood serum testing on the presence of specific antibodies against S. Enteritidis, it can be concluded that cELISA and classic agglutination with O and H antigen methods have a significant place in serological diagnostics of poultry samonelosis, because their application enables detection of the titre of specific antibodies against S. Enteritidis presence in the population of unvaccinated poultry sensitized with antigens of the mentioned causative agent, as well as in unvaccinated animals.
机译：介绍。我们研究的目的是指出针对肠道沙门氏菌亚种肠炎沙门氏菌肠炎沙门氏菌存在的特异性抗体，血清学诊断方法（即竞争性免疫酶检测-cELISA以及O和H沙门氏菌抗原经典凝集方法）的重要性接种和未接种家禽的血清样本中的滴度和滴度检测。材料与方法。在我们的工作中，我们使用了商业竞争性免疫酶测试-cELISA和经典的凝集方法，对肠炎沙门氏菌亚种肠炎血清型肠炎沙门氏菌的O和H抗原进行了检测。比较测试包括177份家禽的血清样本，其中137份来自未接种疫苗，40份来自大多数majority子养殖场的已接种疫苗的个体。结果。在74份血清样本中，通过使用cELISA测试，发现了来自未接种疫苗的羊群的全部待测样本中的54.01％，发现了针对肠炎沙门氏菌的特异性抗体，而通过经典凝集特异性抗体的方法在58个样品中发现了针对O抗原1,9和12的抗体，占42.34％，在61个样品中发现了针对H抗原g和m的特异性抗体，占44,53％。在所有测试过的家禽血清样本中，都通过竞争性cELISA方法以及经典的体细胞O和鞭毛H抗原凝集法，确定了针对肠炎沙门氏菌的特异性抗体的存在。通过经典凝集法和使用ELISA的cELISA方法获得的结果的统计分析，发现很好的依从性（kappa = 0,813）。结论。根据针对肠炎沙门氏菌特异性抗体存在的血清测试结果的比较结果，可以得出结论，因为cELISA和O和H抗原经典凝集法在禽肉沙门氏菌病的血清学诊断中具有重要地位，检测在针对所述致病因子抗原致敏的未接种禽类以及未接种动物中针对肠炎沙门氏菌存在的特异性抗体的滴度。

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