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首页> 外文期刊>Veterinary Sciences >Prevalence of Selected Zoonotic and Vector-Borne Agents in Dogs and Cats on the Pine Ridge Reservation
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Prevalence of Selected Zoonotic and Vector-Borne Agents in Dogs and Cats on the Pine Ridge Reservation

机译：松脊保护区猫狗中某些人畜共患病和媒介传染病媒介的流行

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The prevalence of intestinal parasites and vector-borne agents of dogs and cats in the Pine Ridge Reservation, South Dakota were determined. Fecal samples (84 dogs, 9 cats) were examined by centrifugal floatation and by immunofluorescence assay (FA) for Giardia and Cryptosporidium . PCR was performed on Giardia [beta-giardin (bg), triose phosphate isomerase (tpi), glutamate dehydrogenase genes (gdh)] and Cryptosporidium [heat shock protein-70 gene (hsp)] FA positive samples. Cat sera ( n = 32) were tested for antibodies against Bartonella spp., Toxoplasma gondii , and FIV, and antigens of FeLV and Dirofilaria immitis . Dog sera ( n = 82) were tested for antibodies against T. gondii , Borrelia burgdorferi , Ehrlichia canis , and Anaplasma phagocytophilum and D. immitis antigen. Blood samples (92 dogs, 39 cats) were assessed by PCR for amplification of DNA of Bartonella spp., Ehrlichia spp., Anaplasma spp., haemoplasmas, and Babesia spp. (dogs only). The most significant results were Giardia spp. (32% by FA), Taenia spp. (17.8%) and Cryptosporidium spp. (7.1%). The Giardia isolates typed as the dog-specific assemblages C or D and four Cryptosporidium isolates typed as C. canis . Antibodies against T. gondii were detected in 15% of the dogs. Antibodies against Bartonella spp. and against T. gondii were detected in 37.5% and 6% of the cats respectively. FeLV antigen was detected in 10% of the cats.
机译：确定了南达科他州派恩里奇保留区中狗和猫的肠道寄生虫和媒介传播媒介的患病率。粪便样品（84只狗，9只猫）通过离心浮选和免疫荧光测定（FA）进行贾第鞭毛虫和隐孢子虫的检测。对贾第虫[β-贾第蛋白（bg），磷酸三糖磷酸异构酶（tpi），谷氨酸脱氢酶基因（gdh）]和隐孢子虫[热休克蛋白70基因（hsp）] FA阳性样品进行PCR。对猫血清（n = 32）进行了抗Bartonella spp。，弓形体弓形虫和FIV抗体以及FeLV和Dirofilaria免疫炎抗原的测试。测试了狗血清（n = 82）的抗弓形虫，伯氏疏螺旋体，犬埃里希氏菌，吞噬性无浆细胞和D.免疫炎性抗原的抗体。通过PCR评估血样（92只狗，39只猫）以扩增Bartonella spp。，Ehrlichia spp。，Anaplasma spp。，血肿和Babesia spp的DNA。 （仅限狗）。最显着的结果是贾第虫属。 （FA占32％），Ta虫属。 （17.8％）和隐孢子虫属。 （7.1％）。贾第虫分离株的类型为狗特异性组合C或D，四种隐孢子虫分离株的类型为犬念珠菌。在15％的狗中检出了针对弓形虫的抗体。抗Bartonella spp的抗体。对弓形虫和弓形虫的检出率分别为37.5％和6％。在10％的猫中检测到FeLV抗原。

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