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首页> 外文期刊>Veterinary World >Contamination rate of avian Leukosis viruses among commercial Marek's Disease vaccines in Assiut, Egypt market using reverse transcriptase-polymerase chain reaction
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Contamination rate of avian Leukosis viruses among commercial Marek's Disease vaccines in Assiut, Egypt market using reverse transcriptase-polymerase chain reaction

机译：逆转录酶-聚合酶链反应在埃及阿西乌特市市售的马立克氏病疫苗中禽白血病病毒的污染率

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Avian leukosis viruses ( ALVs ) in poultry may induce a variety of deleterious effects including tumors, increased mortalities, growth retardation and decrease in egg size and production that led to considerable economic losses. The identification of avian leukosis viruses ( ALVs ) in imported Marek’s disease (MD) vaccines has raised concern about transmission of these retroviruses to vaccine recipients esp. poultry breeding stocks, so Egypt as one of importing countries requires freedom of infection with ALVs in such vaccines. Subgroup specific RT-PCR was undertaken on isolated RNA from 13 obtained commercial MD vaccines using six pairs of primers that correspond to envelope glycoprotein gene (gp85) which determines possible contamination with the six ALV subgroups: A, B, C, D, E, and J. The results indicated that RT-PCR assay for ALV-gp85 subgroup-E was positive for eight out of thirteen (61.5%) tested MD vaccines, while primers designed to detect subgroup A and J ALVs were positive for five out of thirteen (38.5%) and two out of thirteen (7.7%) respectively among examined vaccines. No ALVs was detected in 3/13 (23.07%) of commercially examined vaccines by using any of six primer pairs. Finally, the using of RT-PCR assay provides us a new, sensitive approach for identifying ALVs as a contaminant agent that will help greatly in applying this method for equipped labs as a quality control measure for testing delivered MD vaccines before its administration in poultry breeding stocks as well eradication programs through identifying infected birds. Key words : Marek’s disease, vaccine contamination, avian leukosis virus, RT-PCR E-mail: editorveterinaryworld@gmail.com Website: www.veterinaryworld.org.
机译：家禽中的禽白血病病毒（ALV）可能引起多种有害影响，包括肿瘤，死亡率增加，生长迟缓以及蛋大小和产量的下降，从而导致可观的经济损失。进口马立克氏病（MD）疫苗中禽类白血病病毒（ALV）的鉴定引起了人们对将这些逆转录病毒传播给疫苗接受者尤其是人们的担忧。家禽繁殖种群，因此埃及作为进口国之一，要求在此类疫苗中无ALV感染的自由。使用六对对应于包膜糖蛋白基因（gp85）的引物，对来自13种市售MD疫苗的分离RNA进行亚组特异性RT-PCR，以确定六种ALV亚组可能的污染：A，B，C，D，E，结果表明，针对ALV-gp85亚组E的RT-PCR检测，在十三种（61.5％）测试的MD疫苗中呈阳性，而设计用于检测A和J亚型ALV的引物对于十三种中的五种呈阳性（38.5％）和十三分之二（7.7％）的疫苗。通过使用六个引物对中的任何一个，在商业检测的疫苗中有3/13（23.07％）未检测到ALV。最后，RT-PCR分析的使用为我们提供了一种新的灵敏方法，可将ALV鉴定为污染物，这将极大地帮助将该方法用于装备精良的实验室，作为在家禽育种中施用MD疫苗之前对其进行检测的质量控制措施通过识别受感染的鸟类来进行种群和根除计划。关键字：马立克氏病，疫苗污染，禽白血病病毒，RT-PCR电子邮件：editorveterinaryworld@gmail.com网站：www.veterinaryworld.org。

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