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首页> 外文期刊>Virology Journal >Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein
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Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

机译：鸭肠炎病毒（DEV）UL54蛋白是一种新型伴侣，可与DEV UL24蛋白相互作用

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Background UL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication. Results In this paper, to investigate putative UL24-binding proteins and to explore the functional mechanisms of DEV UL24, yeast two-hybrid (Y2H) was carried out, and further verified the interaction between UL24 and partners by co-immunoprecipitation and fluorescence microscopy experiments. Interaction partners of UL24 protein were screened by yeast two-hybrid (Y2H) with the cDNA library of DEV-CHv strain post-infection DEF cells. A novel partner, DEV UL54 protein, was discovered by Y2H screening and bioinformatic. Co-immunoprecipitation experiments suggested that DEV UL24 interacted with UL54 proteins. And distribution of a part of UL54 protein was changed from nucleus to cytoplasm in DF-1 cells of co-subcellular localization experiments which also showed that DEV UL24 interacted with UL54 proteins. Conclusions The interaction between the DEV UL24 and UL54 proteins was discovered for the first time. Thus, DEV UL54 protein as a novel partner interacted with DEV UL24 protein.
机译：背景技术UL24是一种多功能蛋白，在α型疱疹病毒中是保守的，据信在病毒感染和复制中起着重要作用。结果在本文中，为了研究推测的UL24结合蛋白并探讨DEV UL24的功能机制，我们进行了酵母双杂交（Y2H），并通过免疫共沉淀和荧光显微镜实验进一步验证了UL24与伴侣之间的相互作用。 。通过酵母双杂交（Y2H）和DEV-CHv株感染后DEF细胞的cDNA文库筛选UL24蛋白的相互作用伴侣。通过Y2H筛选和生物信息学发现了一种新型伴侣DEV UL54蛋白。免疫共沉淀实验表明DEV UL24与UL54蛋白相互作用。在共亚细胞定位实验的DF-1细胞中，部分UL54蛋白的分布从细胞核改变为细胞质，这也表明DEV UL24与UL54蛋白相互作用。结论首次发现DEV UL24和UL54蛋白之间的相互作用。因此，DEV UL54蛋白作为新型伴侣与DEV UL24蛋白相互作用。

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