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首页> 外文期刊>Virology Journal >Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice
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Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice

机译：含有CpG基序的猪圆环病毒2型核酸疫苗对小鼠的免疫原性

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Background This study aimed at reseaching the immune effect of porcine circovirus type 2 (PCV2) DNA vaccine containing CpG motif on mice. Methods A total of 40 6-week-old female BALB/c mice were randomly divided into four groups which were immunized by 18CpG-pVAX1-ORF2, pVAX1-ORF2, pVAX1 and PBS, respectively, and immunized again 2 weeks later. All mice were challenged with 0.2 mL PCV2 cells virulent strain SD (106.0 TCID50/mL) after 4 weeks. Average daily gain, blood antibody levels, microscopic changes and viremia were detected to estimate the effect of DNA vaccine. Results and Discussion The results showed that compared to those of the control mice, groups immunized with pVAX1-ORF2 and 18CpG-pVAX1-ORF2 could induce PCV2-specific antibodies. The PCV2-specific antibodies level of 18 CpG-pVAX1-ORF2 groups was higher significantly than other groups and decreased slowly along with time. There was no distinct pathological damage and viremia occurring in mice that inoculated with CpG motif DNA vaccines. The results demonstrated that the DNA vaccine containing 18 CpG could build up resistibility immunity and reduce immune organ damage on mice.
机译：背景技术这项研究旨在研究含有CpG基序的猪圆环病毒2型（PCV2）DNA疫苗对小鼠的免疫效果。方法将40只6周龄雌性BALB / c小鼠随机分为4组，分别用18CpG-pVAX1-ORF2，pVAX1-ORF2，pVAX1和PBS免疫，并于2周后再次免疫。 4周后，用0.2mL PCV2细胞毒性株SD（10 6.0 TCID 50 / mL）攻击所有小鼠。检测平均日增重，血液抗体水平，微观变化和病毒血症，以评估DNA疫苗的效果。结果与讨论结果表明，与对照组小鼠相比，用pVAX1-ORF2和18CpG-pVAX1-ORF2免疫的组可以诱导PCV2特异性抗体。 18个CpG-pVAX1-ORF2组的PCV2特异性抗体水平显着高于其他组，并随时间缓慢下降。接种CpG基序DNA疫苗的小鼠中没有发生明显的病理损伤和病毒血症。结果表明，含有18 CpG的DNA疫苗可增强抵抗力免疫力并减少小鼠免疫器官的损伤。

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