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首页> 外文期刊>World Journal of AIDS >Genetic Diversity and Antiretroviral Drug Resistance among Drug-Na?ve HIV-1 Infected Pregnant Women Attending Antenatal Clinics in Abidjan, C?te d'Ivoire
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Genetic Diversity and Antiretroviral Drug Resistance among Drug-Na?ve HIV-1 Infected Pregnant Women Attending Antenatal Clinics in Abidjan, C?te d'Ivoire

机译：在科特迪瓦阿比让参加初生诊所的未受过艾滋病毒HIV-1感染的孕妇中的遗传多样性和抗逆转录病毒药物耐药性

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To clarify the distribution of HIV-1 subtypes and drug resistance-related mutations, we collected and analysed serum from pregnant women who are ARV drug-naive in Abidjan. The prevalence of HIV-1 subtypes and mutations associated with antiretroviral drug resistance among drug-na?ve HIV-1 infected pregnant women was investigated from plasma of 90 young pregnant primigravida. The HIV-1 pol and env genes were amplified by using primers recognizing conserved viral sequences and sequenced by employing BigDye chemistry. Positions 1 - 99 of the PR and 1 - 350 of the RT genes were analyzed for mutations based on the international AIDS society USA panel. In 39 strains which both genes were sequenced including CFR02_AG 30 (76.9%), subtype A 3 (7.7%), CFR06_cpx 2 (5.1%), CFR09_cpx 1 (2.6%), and discordant sequences suggesting the presence of a few number of recombinant involving CRF02-AG and subtype A 3 (7.7%). None of the major drug resistance mutations was detected. The frequent minor mutations associated drug resistance observed were M36I (52%/96.3%), L10I/R/V (19%/35.2%) and L63P (7%/12.9%). The M36I mutation was widespread in all subtypes. Our result demonstrated first a significant level of viral heterogeneity and then only the presence of minor resistance associated mutations. Our study emphasizes the need of HIV sentinel survey in C?te d'Ivoire and shows that pregnant women who are candidates for receiving antiretroviral drug therapies do not contain naturally occurring or preexisting drug resistance mutations. So such drug therapies are likely to be highly effective in this setting.
机译：为了弄清HIV-1亚型的分布和与耐药性相关的突变，我们收集并分析了来自阿比让未接受过ARV药物治疗的孕妇的血清。从90名年轻初生孕妇血浆中调查了初次感染HIV-1的孕妇中HIV-1亚型和与抗逆转录病毒药物耐药性相关的突变的发生率。通过使用识别保守病毒序列的引物扩增HIV-1 pol和env基因，并采用BigDye化学方法进行测序。根据国际爱滋病学会美国小组分析了PR基因1-99位和RT基因1-350位的突变。在这两个基因均已测序的39个菌株中，包括CFR02_AG 30（76.9％），亚型A 3（7.7％），CFR06_cpx 2（5.1％），CFR09_cpx 1（2.6％）和不一致的序列提示存在一些重组体涉及CRF02-AG和亚型A 3（7.7％）。没有检测到主要的耐药性突变。观察到的与耐药性相关的常见轻度突变为M36I（52％/ 96.3％），L10I / R / V（19％/ 35.2％）和L63P（7％/ 12.9％）。 M36I突变广泛存在于所有亚型中。我们的结果首先证明了病毒异质性的显着水平，然后仅是次要抗性相关突变的存在。我们的研究强调了在科特迪瓦进行艾滋病哨兵调查的必要性，并显示了接受抗逆转录病毒药物治疗的孕妇不包含自然发生或先前存在的耐药突变。因此，这种药物疗法可能在这种情况下非常有效。

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