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Validating the use of non-invasively sourced DNA for population genetic studies using pedigree data

机译：使用谱系数据验证将非侵入性来源的DNA用于人群遗传研究

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Non-invasive genetic sampling has provided valuable ecological data for many species – data which may have been unobtainable using invasive sampling methods. However, DNA obtained non-invasively may be prone to increased levels of amplification failure and genotyping error. Utilizing genotype data from 32 pedigreed koalas, this study aimed to validate the reliability of final consensus genotypes obtained using DNA isolated from koala scats. Pedigree analysis, duplicate genotyping, analysis of mismatched loci and tests for null alleles were used to look for evidence of errors. All genetically confirmed parent–offspring relationships were found to follow Mendelian rules of inheritance. Duplicate genotypes matched in all cases and there was no evidence of null alleles. Related individuals always had different 12-marker genotypes having a minimum of three unique loci (in one full sibling pair), a mode of seven unique loci and a maximum of 11 unique loci. This study demonstrates the capacity of DNA recovered from koala scats to provide reliable genotypes that can unequivocally discriminate individuals and infer parentage, provided data are missing from no more than two loci. Validating data obtained using non-invasive sampling is an important step, allowing potential problems to be identified at an early stage.
机译：非侵入性基因采样为许多物种提供了有价值的生态数据，而使用侵入性采样方法可能无法获得这些数据。但是，非侵入性获得的DNA可能容易导致扩增失败和基因分型错误。利用来自32个纯种考拉的基因型数据，本研究旨在验证使用从考拉小便中分离的DNA获得的最终共有基因型的可靠性。家谱分析，重复基因分型，错配基因座分析和无效等位基因测试用于寻找错误的证据。发现所有经遗传学证实的亲子关系都遵循孟德尔的遗传规则。在所有情况下重复的基因型均匹配，并且没有无效等位基因的证据。相关个体总是具有不同的12标记基因型，具有最少3个唯一基因座（在一个完整的同胞对中），7个唯一基因座的模式和最多11个唯一基因座。这项研究表明，从无尾熊中回收的DNA提供可靠的基因型的能力，只要不超过两个基因座的数据缺失，就可以明确地区分个体并推断出亲本。验证使用无创采样获得的数据是重要的一步，可以在早期阶段识别出潜在的问题。

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