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首页> 外文期刊>World Journal of Vaccines >Validation of Automated Dilution and Transfer Methods for Rapid Assessment of Functional Immune Responses to Meningococcal Vaccines
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Validation of Automated Dilution and Transfer Methods for Rapid Assessment of Functional Immune Responses to Meningococcal Vaccines

机译：自动稀释和转移方法对快速评估脑膜炎球菌疫苗功能性免疫反应的验证

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A high-throughput Serum Bactericidal Assay (SBA) was developed to monitor the functional antibody responses to capsular polysaccharide antigens from multiple serotypes of Neisseria meningitidis. This assay measures the ability of an antibody, aided by complement, to mediate killing of bacteria. Functional assays of this type are increasingly used in the quality control arena as potency release tests. Consequently, there has been an enhanced requirement for reproducibility in the performance of this type of assay. Assay validation is, therefore, important to facilitate understanding of the significance of values obtained, and to enable appropriate and informed use of the assay. This study involved the evaluation of the high-throughput serum bactericidal assay including the effects of different dilution and transfer techniques on assay performance. The results presented here demonstrate the repeatability, and the precision and ruggedness of the assay.
机译：开发了一种高通量的血清杀菌试验（SBA），以监测功能性抗体对脑膜炎奈瑟菌多种血清型荚膜多糖抗原的反应。该测定法测量抗体在补体的辅助下介导细菌杀灭的能力。在功能控制领域，这种功能性测定越来越多地用作效能释放测试。因此，对于这种类型的测定的可重复性提出了更高的要求。因此，分析验证对于促进对所获得值的重要性的理解，并使分析能够正确，合理地使用非常重要。这项研究涉及对高通量血清杀菌测定的评估，包括不同稀释和转移技术对测定性能的影响。此处显示的结果证明了测定的可重复性以及精确度和耐用性。

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