• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Yonsei Medical Journal >Prenatal genetic diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative and positive magnetic activated cell sorting
【24h】

Prenatal genetic diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative and positive magnetic activated cell sorting

机译：从母体血中进行产前遗传学诊断：通过负向和正向磁性激活细胞分选法同时检测胎儿有核红细胞的免疫表型和FISH

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fetal nucleated red blood cells (nRBCs) are rare in maternal circulation, but their presence constitutes a potential source of non-invasive prenatal genetic diagnosis. This study was undertaken to establish a non-invasive prenatal genetic diagnosis method using isolated fetal nRBCs. A multi-step method including triple density gradient and magnetic activated cell sorting (MACS) using CD45 and CD71, cytospin centrifugation, K-B staining, and glycophorin A-immuno fluorescence in situ hybridization (GPA-immuno FISH) was performed. The study population included 65 patients from 8 to 41 weeks of gestation, and fetal nRBC was separated from all cases. The number of fetal nRBCs retrieved was 12.8 ± 2.7 in 8 to 11 gestational weeks, 15.2 ± 6.5 in 12 to 18 gestational weeks, 16.4 ± 6.5 in 19 to 23 gestational weeks, 10.6 ± 3.2 in 24 to 28 gestational weeks, and 5.5 ± 1.9 in 35 to 41 gestational weeks: the mean number of nRBCs collected from 20 ml of maternal peripheral blood was 13.7 ± 6.2. The highest value of yield was 45.6% from 12 to 18 weeks gestation. The fetal sex determination confirmed by amniocentesis or chorionic villus sampling showed 100% sensitivity and 91.7% specificity for males; 91.7% sensitivity and 100% specificity for females. We showed that fetal cells can be reliably enriched from maternal blood and that they can be used for detecting specific chromosomes by FISH with a specificity superior to current non-invasive methods.
机译：胎儿有核红细胞（nRBC）在母体循环中很少见，但它们的存在构成了非侵入性产前遗传学诊断的潜在来源。进行这项研究以建立使用分离的胎儿nRBC的非侵入性产前遗传诊断方法。进行了包括三重密度梯度和使用CD45和CD71的磁激活细胞分选（MACS），细胞旋转离心，K-B染色和糖蛋白A-免疫荧光原位杂交（GPA-免疫FISH）的多步骤方法。研究人群包括65名妊娠8至41周的患者，胎儿nRBC与所有病例均分开。在8至11个孕周中回收的胎儿nRBC数为12.8±2.7，在12至18个孕周中为15.2±6.5，在19至23个孕周中为16.4±6.5，在24至28个孕周中为10.6±3.2，以及5.5±在妊娠35至41周中有1.9个：从20 ml孕妇外周血中收集到的nRBC的平均数为13.7±6.2。妊娠12至18周时的最高产值为45.6％。通过羊膜穿刺术或绒毛膜绒毛取样证实的胎儿性别测定对男性的敏感性为100％，特异性为91.7％;女性敏感性为91.7％，特异性为100％。我们证明胎儿细胞可以可靠地从母体血液中富集，并且可以通过FISH用于检测特定染色体，其特异性优于目前的非侵入性方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号