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Genetic diversity among natural populations of Mandevilla velutina

机译：扁桃自然种群的遗传多样性

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Mandevilla velutina (Mart. Ex Stadelm) Woodson (Apocynaceae) is an endemic species in the Cerrado (Brazilian Savannah), whose alcoholic extracts and root infusions are extensively used in the popular medicine to treat inflammatory diseases and against snake bites. Currently, this species has been pointed out as crucial in conservation programs. Therefore, studies on its genetic diversity, as well as the development of methodology for in vitro conservation in germplasm banks are imperative to avoid its extinction. The objective of this work was to investigate the intra- and inter-populational genetic variability of M. velutina to enhance the representativeness of germplasm banks. To this aim, we used RAPD molecular markers. The analysis of molecular variance (AMOVA) indicated that the intra-populational (81.25%) was higher than the inter-populational (18.75%) variability, which was confirmed by the Nei's Diversity Index. The PHIst (0.188) and qP (0.1586) values of genetic variation indicated high population structuring. There seems to be no direct correlation between geographic distances and genetic similarity among the three studied populations.
机译：曼德维拉velutina（Mart。Ex Stadelm）伍德森（Apocynaceae）是塞拉多（巴西大草原）的特有物种，其酒精提取物和根部浸剂广泛用于流行药物中，以治疗炎性疾病和抗蛇咬伤。目前，已经指出该物种在保护计划中至关重要。因此，必须对其遗传多样性进行研究，并发展种质库中体外保存的方法，以避免其灭绝。这项工作的目的是调查velutina的种群内和种群间的遗传变异，以增强种质库的代表性。为此，我们使用了RAPD分子标记。分子变异分析（AMOVA）表明，种群内变异（81.25％）高于种群间变异（18.75％），这一点已通过Nei's多样性指数得到了证实。遗传变异的PHIst（0.188）和qP（0.1586）值表明种群结构较高。在这三个研究人群中，地理距离和遗传相似性之间似乎没有直接关联。

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