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Results of in vitro chemotherapy of apple cv. Fragrance – Short communication

机译：苹果简历的体外化学疗法的结果。香气–沟通短促

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The effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial field-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with well-developed leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the first chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. The success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity.
机译：利巴韦林化学疗法对受感染苹果cv的体外生长植物中的梨果病毒的清除作用。香味已被调查。 ELISA和RT-PCR测试的结果证明了在该苹果的最初田间生长的树中混合感染了苹果茎点病毒（ASPV），苹果绿叶斑病毒（ACLSV）和苹果茎沟病毒（ASGV）品种获得的具有良好发育的叶片的主动生长的体外芽和芽尖随后用于利巴韦林的化学疗法。尝试通过较低浓度20 mg / l的病毒唑完全消除病毒是没有成功的。但是，从ASPV和ASGV消毒的一种美罗尼酮（FR1R20）的体外植物仅在第一个化疗周期后才被ACLSV感染，然后在病毒唑浓度较高100 mg / l的培养基上重复处理。在第二轮之后，用ACLSV消除100 mg / l的病毒唑的化学疗法的成功率为76％。在两个化疗周期（20 mg / l和100 mg / l）的过程中，体外植物均未显示出植物毒性症状。

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