Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation

I. Ajalin; F. Kobza; J. Dole?el

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Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation

机译：中提琴×wittrockiana Gams中染色体倍数植物的倍性鉴定。 M 1代

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The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola × wittrockiana Gams. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the M1-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.

机译：这项研究的目的是开发一种生产中提琴×wittrockiana Gams多倍体M1代植物的方案。比较了秋水仙碱治疗的两种变体的效率。通过筛选气孔大小，叶片索引值（叶片长度/宽度）以及M1代的其他形态特征，可以实现对新倍性水平的早期检测。通过流式细胞仪（FCM）进行新倍性水平的二次筛选。通过FCM成功鉴定了六倍体，非整倍体和混合倍体植物。

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《Horticultural Science》 |2002年第1期|共6页
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I. Ajalin; F. Kobza; J. Dole?el;
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Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation

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