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首页> 外文期刊>Horticulture research. >Wide transcriptional investigation unravel novel insights of the on-tree maturation and postharvest ripening of ‘Abate Fetel’ pear fruit
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Wide transcriptional investigation unravel novel insights of the on-tree maturation and postharvest ripening of ‘Abate Fetel’ pear fruit

机译：广泛的转录研究揭示了“ Abate Fetel”梨果实在树上成熟和采后成熟的新颖见解

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To decipher the transcriptomic regulation of the on-tree fruit maturation in pear cv. 'Abate Fetel', a RNA-seq transcription analysis identified 8939 genes differentially expressed across four harvesting stages. These genes were grouped into 11 SOTA clusters based on their transcriptional pattern, of which three included genes upregulated while the other four were represented by downregulated genes. Fruit ripening was furthermore investigated after 1 month of postharvest cold storage. The most important variation in fruit firmness, production of ethylene and volatile organic compounds were observed after 5 days of shelf-life at room temperature following cold storage. The role of ethylene in controlling the ripening of 'Abate Fetel' pears was furthermore investigated through the application of 1-methylcyclopropene, which efficiently delayed the progression of ripening by reducing fruit softening and repressing both ethylene and volatile production. The physiological response of the interference at the ethylene receptor level was moreover unraveled investigating the expression pattern of 12 candidate genes, initially selected to validate the RNA-seq profile. This analysis confirmed the effective role of the ethylene competitor in downregulating the expression of cell wall (PG) and ethylene-related genes (ACS, ACO, ERS1, and ERS2), as well as inducing one element involved in the auxin signaling pathway (Aux/IAA), highlighting a possible cross-talk between these two hormones. The expression patterns of these six elements suggest their use as molecular toolkit to monitor at molecular level the progression of the fruit on-tree maturation and postharvest ripening.
机译：破译梨果树上成熟果实的转录组调控。 RNA-seq转录分析“ Abate Fetel”确定了在四个收获阶段差异表达的8939个基因。这些基因根据其转录方式分为11个SOTA簇，其中三个包括上调的基因，而其他四个则由下调的基因代表。采后冷藏1个月后进一步调查了果实的成熟度。冷藏后，在室温下放置5天后，观察到水果硬度，乙烯和挥发性有机化合物产生的最重要变化。此外，还通过应用1-甲基环丙烯研究了乙烯在控制'Abate Fetel'梨成熟中的作用，该过程通过减少果实的软化并抑制乙烯和挥发性物质的产生有效地延迟了成熟的进程。此外，还对乙烯受体水平上的干扰的生理反应进行了调查，研究了12个候选基因的表达模式，这些候选基因最初被选择来验证RNA-seq图谱。该分析证实了乙烯竞争者在下调细胞壁（PG）和乙烯相关基因（ACS，ACO，ERS1和ERS2）的表达以及诱导生长素信号传导途径（Aux / IAA），强调了这两种激素之间可能的相互干扰。这六个元素的表达模式表明它们可用作分子工具包，以在分子水平上监测果实在树上成熟和收获后成熟的进程。

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