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首页> 外文期刊>Dental Journal: Majalah Kedokteran Gigi >The effects of Anadara granosa shell-Stichopus hermanni on bFGF expressions and blood vessel counts in the bone defect healing process of Wistar rats
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The effects of Anadara granosa shell-Stichopus hermanni on bFGF expressions and blood vessel counts in the bone defect healing process of Wistar rats

机译：Anadara granosa shell-Stichopus hermanni对Wistar大鼠骨缺损愈合过程中bFGF表达和血管计数的影响

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Background: Bone damage can be caused by various factors with treatment usually involving graft materials being applied to the defective area. Moreover, in the bone defect healing process, blood vessels are also considered to be an important energy source for cell proliferation. One of the angiogenic factors playing an important role in blood vessel formation is basic fibroblast growth factor (bFGF). Furthermore, synthesized hydroxyapatite derived from Anadara granosa (AG) shells constitutes one of the potential materials for use in bone graft. The gold sea cucumber genus Stichopus hermanni (SH) possesses the ability to stimulate endothelial progenitor cells inducing bFGF. Purpose: This study aims to investigate the effects of AG shells and SH on bFGF expressions and blood vessel counts within the bone healing process. Methods: Twenty four male Wistar rats were divided into three groups, namely: a control group (C), a treatment group was administered with blood cockle shell (AG), and a treatment group with blood cockle shell and golden sea cucumber (AG+SH). Defects were made on their femurs measuring half the diameter of a circular, no. 018. bur These rats were subsequently sacrificed on day 7 after surgery. The expressions of bFGF were measured by means of IHC technique, while the number of blood vessels was quantified using HE technique. The resulting data was subjected to statistical analysis using an Anova test followed by an LSD test (p < 0.05). Results: The one-way Anova test results combined with those of an LSD test showed there to be significant differences in bFGF expressions and blood vessel counts between the control group (K) and the treatment group (AG) as well as between the treatment group (AG) and the treatment group (AG+SH). Conclusions: A combination of Anadara granosa shell and Stichopus hermanni can increase the expression of bFGF and the number of blood vessels on day 7 during the bone healing process in Wistar rats.
机译：背景：骨骼损伤可能是由多种因素引起的，通常需要在缺陷区域使用移植材料进行治疗。此外，在骨缺损愈合过程中，血管也被认为是细胞增殖的重要能量来源。在血管形成中起重要作用的血管生成因子之一是碱性成纤维细胞生长因子（bFGF）。此外，衍生自Anadara granosa（AG）贝壳的合成羟基磷灰石构成了用于骨移植的潜在材料之一。金海参属Stichopus hermanni（SH）具有刺激内皮祖细胞诱导bFGF的能力。目的：本研究旨在研究AG壳和SH对骨愈合过程中bFGF表达和血管计数的影响。方法：将24只雄性Wistar大鼠分为三组，分别为：对照组（C），治疗组给予血蛤壳（AG），治疗组给予血蛤壳和金海参（AG + SH）。股骨缺损测量为圆形直径的一半，没有。 018.bur这些小鼠随后在手术后的第7天被处死。用IHC技术测定bFGF的表达，用HE技术定量血管数。使用Anova检验和LSD检验对所得数据进行统计分析（p <0.05）。结果：单向Anova试验结果与LSD试验结果相结合，显示对照组（K）与治疗组（AG）以及治疗组之间bFGF表达和血管计数存在显着差异（AG）和治疗组（AG + SH）。结论：Anastara granosa shell和Stichopus hermanni的组合可以在Wistar大鼠骨骼愈合的第7天增加bFGF的表达和血管数量。

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