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A Study on the Production of Bioethanol from Portieria hornemannii Seaweed

机译:霍恩曼氏海藻海藻生产生物乙醇的研究

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Bioethanol production was investigated in red algae Portieria hornemannii which belongs to the family Rhizophyllidaceae. Saccharomyces cerevisiae was cultured on Potato Dextrose Agar (PDA) media that had been sterilized at a temperature of 121°C and at a pressure of 1 atm for 15 min. It was incubated at 30°C for 3 days. Microbes on PDA media as culture stock was regenerated on the Yeast Extract Peptone Dextrose (YPD) media at 30°C for 24 h before mixing with hydrolysate. Reducing sugar was determined by 3,5-dinitrosalicylic acid (DNS) method. The seaweed hydrolysate from 60 g of seaweed gel (15 g of seaweed) yielded alcohol maximum in fermentate using S. cerevisiae as fermentation agent. Fermentation to produce alcohol was carried around 36-168 h. Bread yeast S. cerevisiae was found suitable for the bioethanol fermentation of seaweed hydrolysate, and gave an alcohol content of fermentate after 20-40 h of fermentation at room temperature.
机译:在红藻Portieria hornemannii中研究了生物乙醇的生产,该藻属于Rhizophyllidaceae科。将啤酒酵母在马铃薯葡萄糖琼脂(PDA)培养基上培养,该培养基已在121℃的温度和1atm的压力下灭菌15分钟。将其在30℃下孵育3天。 PDA培养基上的微生物作为培养物在30℃下于酵母提取蛋白ept右旋糖(YPD)培养基上再生24小时,然后与水解产物混合。还原糖通过3,5-二硝基水杨酸(DNS)方法测定。使用酿酒酵母作为发酵剂,从60克海藻凝胶(15克海藻)中得到的海藻水解产物在发酵物中的酒精含量最高。发酵产生醇约36-168小时。发现面包酵母酿酒酵母适于海藻水解产物的生物乙醇发酵,并且在室温发酵20-40h后产生发酵物的醇含量。

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