...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Der Pharma Chemica: journal for medicinal chemistry, pharmaceutical chemistry and computational chemistry >Inhibitory kinetics of enterolactone on mushroom tyrosinase
【24h】

Inhibitory kinetics of enterolactone on mushroom tyrosinase

机译：肠内酯对蘑菇酪氨酸酶的抑制动力学

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tyrosinase (EC 1.14.18.1) is a key enzyme in post-harvest browning of fruit and melanin formation in human skin. Tyrosinase inhibitors are important in agricultural and cosmetic chemistry. In the present study, the inhibitory kinetics of enterolactone on themonophenolase and diphenolase activities of mushroom tyrosinasewere investigated using the kinetic method of substrate reaction. The results showed that enterolactone inhibited themonophenolase and diphenolase activities of mushroom tyrosinase, with IC50 values of 0.42 and 0.124 mM, respectively. The inhibition kinetics showed that enterolactone displayed non-competitive mechanism. The inhibition constants were determined to be 0.8315 and 0.6956mM, respectively. Furthermore, enterolactone exerted a potent inhibitory effect on intracellular melanin formation in B16/F10 murine melanoma cells and did not cause cytotoxicity. These results provide a comprehensive understanding of the inhibitory mechanisms underlying enterolactoneactivity and its inhibition of melanin formation, suggesting the potential benefits of using this compound.
机译：酪氨酸酶（EC 1.14.18.1）是水果收获后褐变和人体皮肤黑色素形成的关键酶。酪氨酸酶抑制剂在农业和化妆品化学中很重要。本研究采用底物反应动力学方法研究了肠内酯对蘑菇酪氨酸酶单酚酶和双酚酶活性的抑制动力学。结果表明，肠内酯能抑制蘑菇酪氨酸酶的单酚酶和双酚酶活性，IC50值分别为0.42和0.124 mM。抑制动力学表明肠内酯显示出非竞争性机理。测定的抑制常数分别为0.8315和0.6956mM。此外，肠内酯对B16 / F10鼠黑色素瘤细胞中细胞内黑色素的形成具有有效的抑制作用，并且不会引起细胞毒性。这些结果提供了对肠内酯活性的抑制机制及其对黑色素形成的抑制作用的全面理解，表明使用该化合物的潜在益处。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号