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首页> 外文期刊>Dhaka University Journal of Pharmaceutical Sciences >Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations
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Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

机译：穿心莲穿心莲内酯中穿心莲内酯的鉴定和定量。 RP-HPLC法检测nees及其市场准备的标准化

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Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 μg/mL in the solution of amorphous residue (50 μg/mL). After quantitative determination of andrographolide, standardization of its market preparations was accomplished using the same RP-HPLC method. The estimated % potencies of andrographolide compared to reference standard in six market preparations were found to be 97.56, 98.37, 98.21, 95.60, 98.91 and 96.40.Dhaka Univ. J. Pharm. Sci. 14(1): 71-78, 2015 (June)
机译：穿心莲（Burf。f。）Wall。 ex Nees，通常称为Kalmegh，在草药和传统系统中广泛用作抗疟药。穿心莲内酯是植物中主要的三萜类化合物，并负责其治疗活性。穿心莲内酯的鉴定和定量通过各种分光光度法和色谱分析来确定。用甲醇提取植物样品。通过化学方法，TLC，UV-Vis，FT-IR和LCMS / MS分析，分析了从提取物中获得的无定形残留物，以鉴定穿心莲内酯。通过简单的反相HPLC方法，使用C18色谱柱，以水和甲醇（35:65）的混合物作为流动相，流速为0.7 mL / min，估计浓度，对穿心莲内酯含量进行定量估计。在无定形残留物（50μg/ mL）溶液中，穿心莲内酯的含量为38.36±0.42μg/ mL。穿心莲内酯的定量测定后，使用相同的RP-HPLC方法完成其市场准备的标准化。在六种市售制剂中，穿心莲内酯与参考标准品相比的估计效力百分比为97.56、98.37、98.21、95.60、98.91和96.40。 J.药物科学14（1）：71-78，2015（六月）

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