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首页> 外文期刊>Diagnostic pathology >Protective and antidiabetic effects of extract from Nigella sativa on blood glucose concentrations against streptozotocin (STZ)-induced diabetic in rats: an experimental study with histopathological evaluation
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Protective and antidiabetic effects of extract from Nigella sativa on blood glucose concentrations against streptozotocin (STZ)-induced diabetic in rats: an experimental study with histopathological evaluation

机译：苜蓿提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠血糖浓度的保护和抗糖尿病作用：具有组织病理学评估的实验研究

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Background Diabetes in humans induces chronic complications such as cardiovascular damage, cataracts and retinopathy, nephropathy and polyneuropathy. The most common animal model of human diabetes is streptozotocin (STZ)-induced diabetes in the rat. The present study investigated the effects of Nigella sativa hydroalcholic extract on glucose concentrations in streptozotocin (STZ) diabetic rats. Methods In this study Twenty-five Wister-Albino rats (aged 8-9 weeks and weighing 200-250 g) were tested. Rats were divided into five experimental groups (control, untreated STZ-diabetic (60 mg/kg B.W., IP), treated STZ-diabetic with hydroalcholic extract of Nigella Sativa (NS) (5 mg/kg B.W, IP), treated STZ-diabetic with hydroalcholic extract of NS (10 mg/kg B.W., IP) and treated STZ-diabetic with hydroalcholic extract of NS (20 mg/kg B.W., IP and 32 days were evaluated to assess its effect on fasting blood glucose (FBG), and in different groups fasting blood glucose (FBG) and body weight (BW) were measured in the particular days (1, 16 and 32). At the end of the study, the animals were fasted overnight, anaesthetized with an intraperitoneal injection of sodium pentobarbital (60 mg/kg), and sacrificed for obtaining tissues samples (liver, pancreases). The number of islets and cells were counted and the islet diameters were determined by calibrated micrometer. The glycogen content in the liver was examined by Periodic Acid-Schiff (PAS) staining. Results Treatment with NS (5 mg/kg b.w.) markedly increased BW gain and the FBG level was significantly (p<0.001) reduced when compared to the control. Histopathological examination showed that the NS (5 mg/kg b.w.) partially recovered hepatic glycogen content and protected the great deal of the pancreatic islet cells. The number of islets, cells and islets diameter were found statistically significant when compared to the control (p<0.01, p<0.05). Conclusions Higher doses of NS did not exhibit any therapeutic effect. These results showed that hydroalcholic extract of NS at low doses has hypoglycemic effect and ameliorative effect on regeneration of pancreatic islets and may be used as a therapeutic agent in the management of diabetes mellitus. The hypoglycemic effect observed could be due to amelioration of β-cell, thus leading to increased insulin levels. Consequently, N. sativa may prove clinically useful in the treatment of diabetics and in the protection of β-cells against streptozotocin. Virtual slide The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1845133011104231 webcite
机译：背景技术人的糖尿病诱发慢性并发症，例如心血管损伤，白内障和视网膜病，肾病和多发性神经病。人类糖尿病最常见的动物模型是链脲佐菌素（STZ）诱导的大鼠糖尿病。本研究调查了黑鬼菜水醇提取物对链脲佐菌素（STZ）糖尿病大鼠葡萄糖浓度的影响。方法在本研究中，对25只Wister-Albino大鼠（年龄8-9周，体重200-250 g）进行了测试。将大鼠分为五个实验组（对照组，未经治疗的STZ糖尿病患者（60 mg / kg体重，IP），用Nigella Sativa（NS）的水溶胆汁提取物治疗的STZ糖尿病患者（5 mg / kg体重，IP），经治疗的STZ-评估了糖尿病患者的水合胆汁提取物（10 mg / kg体重，腹腔注射）和经治疗的STZ糖尿病患者的水合胆汁提取物（20 mg / kg体重，腹腔注射）和32天，以评估其对空腹血糖（FBG）的影响，在不同的组中，分别在特定的日子（1、16和32）测量空腹血糖（FBG）和体重（BW）。在研究结束时，将动物禁食过夜，并通过腹膜内注射钠对其进行麻醉戊巴比妥（60 mg / kg），处死以获取组织样品（肝，胰），计数胰岛和细胞的数目，并通过校准的千分尺确定胰岛的直径，并用高碘酸法检查肝脏中的糖原含量， Schiff（PAS）染色结果用NS（5 mg / kg bw）进行治疗mar与对照组相比，BW增益显着增加，而FBG水平显着降低（p <0.001）。组织病理学检查表明，NS（5mg / kg b.w.）部分地恢复了肝糖原含量并保护了大量的胰岛细胞。与对照组相比，发现胰岛的数量，细胞和胰岛直径具有统计学意义（p <0.01，p <0.05）。结论较高剂量的NS并没有任何治疗作用。这些结果表明，低剂量的NS的水醇提取物具有降血糖作用和对胰岛再生的改善作用，并且可以用作治疗糖尿病的治疗剂。观察到的降血糖作用可能是由于β细胞的改善，从而导致胰岛素水平升高。因此，苜蓿猪笼草在临床上可被证明可用于治疗糖尿病和保护β细胞抵抗链脲佐菌素。虚拟幻灯片可在此处找到本文的虚拟幻灯片：http://www.diagnosticpathology.diagnomx.eu/vs/1845133011104231网站

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