首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Anther Culture Potential of Linseed ( Linum usitatissimumL.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation
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Anther Culture Potential of Linseed ( Linum usitatissimumL.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation

机译:亚麻子(Linum usitatissimumL。)的花药培养潜力:基因型和预处理对愈伤组织形成和分化的影响

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This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus induction rate both on solid and in liquid medium. The cultivar Blue-Chip gave, on average, the maximum (3.67%) callus induction rate on solid medium and the cultivar Antares gave, on average, the maximum (3.35%) callus induction rate in liquid medium. The cultivar Norman produced callus on solid medium but did not produce any callus in liquid medium. On the other hand, the cultivar McGregor did not produce any callus in either solid or liquid medium. Pre-treatment of flower buds with cold significantly reduced callus induction rate by about four fold compared with the no pre-treatment on solid medium but increased (not statistically significant) callus induction rate by 25.8% in liquid medium. Although some callus induction was achieved in both on solid and in liquid media, no differentiation was observed when calli were transferred to regeneration medium.
机译:这项研究与亚麻籽单倍体的生产有关。在固体和液体培养基上培养了八种基因型的花药。发现在两种培养基中愈伤组织的诱导率均低,并且栽培品种在固体和液体培养基上均显着影响愈伤组织的诱导率。蓝筹品种在固体培养基上的平均愈伤组织诱导率最高(3.67%),而Antares品种在液体培养基上的最大愈伤组织诱导率平均(3.35%)。诺曼品种在固体培养基上产生愈伤组织,但在液体培养基上不产生愈伤组织。另一方面,McGregor品种在固体或液体培养基中均未产生愈伤组织。与未在固体培养基上进行预处理相比,冷处理花芽显着降低了愈伤组织诱导率约4倍,但在液体培养基上则使愈伤组织诱导率提高了25.8%(无统计学意义)。尽管在固体和液体培养基上均实现了一定程度的愈伤组织诱导,但是当将愈伤组织转移至再生培养基上时未观察到分化。

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