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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Obtention of in vitro Haploid Plants From in situ Induced Haploid Embryos in Cucumber (Cucumis sativus L.)
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Obtention of in vitro Haploid Plants From in situ Induced Haploid Embryos in Cucumber (Cucumis sativus L.)

机译：从黄瓜（Cucumis sativus L.）原位诱导的单倍体胚中获得体外单倍体植物

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In this study the obtention of in vitro haploid plants from haploid embryos induced by pollination with irradiated pollen was investigated. The haploid embryos of four cucumber genotypes obtained in different season of the year were cultured on the E20A medium under aseptic condition in 1992-1994. The percentage of embryos that turned into plantlets, duration needed for plant formation, and in situ plantlet development were investigated. Also, the micropropagation possibility with clonning and propagation coefficient of plantlets were determined. The ability of embryos at advanced-stages to form plants was found to be higher (60 % in the first year and 80 % in the second year) than the ones at globular stage. These embryos also produced haploid plants rapidly (in 3.5-day). A higher number of haploid plants was derived from the embryos that were cultured from May to September than from the ones that were cultured in other periods of a year. A maximum of 82 % of the embryos cultured in June produced haploid plants in the second year. The plantlets developed well on the media used and could be cloned with ease. The interval between successive clonings was approximately 30 days. The number of micro cuttings per plant ranged from 3 to 12. Beside that fact that the number of haploid plants per fruit was not high enough, total 190 haploid plants were obtained in four genotypes throughout the study.
机译：在这项研究中，研究了用辐照花粉授粉诱导的单倍体胚中体外单倍体植物的形成。在1992-1994年在无菌条件下在E20A培养基上培养了一年中不同季节获得的四种黄瓜基因型的单倍体胚。研究了变成小植株的胚的百分比，植物形成所需的持续时间以及原位的小植株发育。另外，还确定了小植株具有克隆和繁殖系数的微繁殖可能性。发现处于晚期阶段的胚胎形成植物的能力要比处于球形阶段的胚胎更高（第一年为60％，第二年为80％）。这些胚胎还可以快速（在3.5天之内）产生单倍体植物。从5月至9月培养的胚产生的单倍体植物数量要多于一年中其他时期的胚。在第二年的六月中，最多培养了82％的胚产生了单倍体植物。幼苗在所用培养基上发育良好，可以轻松克隆。连续克隆之间的间隔约为30天。每株植物的微插条数量范围为3至12。除了每个水果的单倍体植物数量不够高的事实外，在整个研究中共获得了四种基因型的190种单倍体植物。

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