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首页> 外文期刊>Disease markers >The Association of Seminal Plasma Antioxidant Levels and Sperm Chromatin Status with Genetic Variants ofGSTM1andGSTP1(Ile105Val and Ala114Val) in Infertile Men with Oligoasthenoteratozoospermia
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The Association of Seminal Plasma Antioxidant Levels and Sperm Chromatin Status with Genetic Variants ofGSTM1andGSTP1(Ile105Val and Ala114Val) in Infertile Men with Oligoasthenoteratozoospermia

机译：精子少精症男性不育症患者精浆抗氧化水平和精子染色质状态与GSTM1和GSTP1（Ile105Val和Ala114Val）遗传变异的关系

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In this study we aimed to examine the effects of genetic variants ofGSTM1andGSTP1(Ile105Val and Ala114Val) on GST activity, seminal oxidative stress and sperm chromatin status in infertile men with oligoasthenoteratozoospermia (OAT). The study population (n= 121) consisted of 95 infertile men with OAT and 26 controls with normozoospermia. Multiplex polymerase chain reaction (PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were utilized to detect the aforesaid genetic variants. We measured GST activity and total antioxidant capacity (TAC) of seminal plasma by spectrophotometry. Sperm chromatin integrity and maturity were assessed using toluidine blue and chromomycin A3(CMA3-positive sperm) staining, respectively. The analysis showed that subgroups of GSTM1 null and GSTP1 C/T+T/T genotypes in comparison with GSTM1 present and GSTP1 wild type (C/C) genotypes did not have statistically significant differences in both OAT or normozoospermic men considering sperm concentration and motility, percentage of CMA3-positive sperm, seminal plasma TAC, sperm chromatin integrity and GST activity. Thus, the findings of our study suggest that there are no significant associations betweenGSTM1andGSTP1polymorphisms and sperm parameters at conventional or at molecular levels including OS status, sperm chromatin integrity or maturity in Iranian infertile men with OAT and normozoospermia. However, these polymorphisms could be related to the fertility status of the studied population but not evaluated in this study.
机译：在这项研究中，我们旨在检查GSTM1和GSTP1的遗传变异（Ile105Val和Ala114Val）对不育男性少精症男性精子（OAT）的GST活性，精子氧化应激和精子染色质状态的影响。研究人群（n = 121）由95名OAT不育男性和26名正常精子症对照组组成。利用多重聚合酶链反应（PCR）和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测上述遗传变异。我们通过分光光度法测量了精浆的GST活性和总抗氧化能力（TAC）。分别使用甲苯胺蓝和嗜铬菌素A3（CMA3阳性精子）染色评估精子染色质的完整性和成熟度。分析显示，与考虑存在的GSTM1和GSTP1野生型（C / C）基因型相比，GSTM1 null和GSTP1 C / T + T / T基因型的亚组在OAT或正常精子症的男性中，考虑到精子浓度和运动性均无统计学差异。 ，CMA3阳性精子百分比，精浆TAC，精子染色质完整性和GST活性。因此，我们的研究结果表明，在具有OAT和正常精子症的伊朗不育男性中，在常规或分子水平上，GSTM1和GSTP1多态性与精子参数之间没有显着关联，包括OS状态，精子染色质完整性或成熟度。但是，这些多态性可能与所研究人群的生育状况有关，但未在本研究中进行评估。

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