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首页> 外文期刊>Diseases of Aquatic Organisms >Experimental bath infection with Flavobacterium psychrophilum, inducing typical signs of rainbow trout Oncorhynchus mykiss fry syndrome
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Experimental bath infection with Flavobacterium psychrophilum, inducing typical signs of rainbow trout Oncorhynchus mykiss fry syndrome

机译：实验性精神病杆菌感染浴浴，诱发虹鳟鱼Oncorhynchus mykiss鱼苗综合征的典型体征

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ABSTRACT: Flavobacterium psychrophilum infection in salmonid fish, known as rainbow trout fry syndrome (RTFS) or bacterial coldwater disease (BCWD), is widespread in fish farms and natural waters. Despite many studies in which attempts at infection were made, an adequate method of infection has not yet been established. In this study, we evaluated a bath infection method in which we used bacteria at different stages of growth in the infection of rainbow trout Oncorhynchus mykiss. Rainbow trout with a mean body weight of 1.3 or 5.6 g, respectively, were infected by immersion in a bacterial suspension at different stages of growth (18 to 66 h shaking culture at 15°C). The fish immersed in a logarithmic phase culture showed higher mortality than those in other culture phases. Indeed, 1.3 and 5.6 g fish showed typical clinical signs including ulcerative tissue of the trunk and lack of caudal fin edge. F. psychrophilum was detected by immunohistochemistry (IHC) in these tissue samples. These results indicate that experimental bath infection using a logarithmic phase bacterial solution is the most appropriate method for studies of infectious mechanisms.
机译：摘要：鲑鱼中的 Psychoophilum 感染，被称为虹鳟鱼苗综合征（RTFS）或细菌性冷水病（BCWD），在养鱼场和天然水域中普遍存在。尽管进行了许多尝试进行感染的研究，但尚未建立适当的感染方法。在这项研究中，我们评估了一种浴感染方法，该方法在虹鳟 Oncorhynchus mykiss 的感染中使用了处于不同生长阶段的细菌。在不同的生长阶段（在15°C摇动培养18至66 h），将平均体重分别为1.3或5.6 g的虹鳟鱼浸入细菌悬浮液中进行感染。浸泡在对数阶段养殖中的鱼显示出比其他养殖阶段更高的死亡率。实际上，1.3克和5.6克鱼显示出典型的临床体征，包括躯干溃疡组织和尾鳍边缘不足。 F。通过免疫组织化学（IHC）在这些组织样本中检测到了嗜冷菌。这些结果表明，使用对数相细菌溶液进行实验性浴感染是研究感染机制的最合适方法。

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