• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Drug Design, Development and Therapy >Skin regeneration in deep second-degree scald injuries either by infusion pumping or topical application of recombinant human erythropoietin gel
【24h】

Skin regeneration in deep second-degree scald injuries either by infusion pumping or topical application of recombinant human erythropoietin gel

机译：通过输注泵或局部应用重组人促红细胞生成素凝胶局部深层烫伤引起的皮肤再生

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: Large doses of recombinant growth factors formulated in solution form directly injected into the body is usual clinical practice in treating second-degree scald injuries, with promising results, but this approach creates side effects; furthermore, it may not allow appropriate levels of the factor to be sensed by the target injured tissue/organ in the specific time frame, owing to complications arising from regeneration. In this research, two delivery methods (infusion pumping and local topical application) were applied to deliver recombinant human erythropoietin (rHuEPO) for skin regeneration. First, rHuEPO was given in deep second-degree scald injury sites in mice by infusion pump. Vascularization was remarkably higher in the rHuEPO pumping group than in controls. Second, local topical application of rHuEPO gel was given in deep second-degree scald injury sites in rats. Histological analysis showed that epithelialization rate was significantly higher in the rHuEPO gel-treated group than in controls. Immunohistochemical studies showed that the rHuEPO gel-treated group showed remarkably higher expression of skin regeneration makers than the control group. An accurate method for visualization and quantification of blood vessel networks in target areas has still not been developed up to this point, because of technical difficulties in detecting such thin blood vessels. A method which utilizes a series of steps to enhance the image, removes noise from image background, and tracks the vessels edges for vessel segmentation and quantification has been used in this study. Using image analysis methods, we were able to detect the microvascular networks of newly formed blood vessels (less than 500 μm thickness), which participate in the healing process, providing not only nutrition and oxygen to grow tissues but also necessary growth factors to grow tissue cells for complete skin regeneration. The rHuEPO-treated group showed higher expression of stem cell markers (CD?31, CD 90, CD 71, and nestin), which actively contribute to in-wound-healing processes for new hair follicle generation as well as skin regeneration. Collectively, both rHuEPO group pumping into the systemic circulation system, and injection into the local injury area, prompted mice and rats to form new blood vessel networks in scald injury sites, which significantly participate in the scald healing process. These results may lead to the development of novel treatments for scald wounds.
机译：【摘要】大剂量以溶液形式配制的重组生长因子直接注射入体内是治疗二度烫伤的常规临床方法，效果可喜，但这种方法产生副作用。此外，由于再生引起的并发症，它可能不允许目标损伤组织/器官在特定的时间范围内检测到适当水平的因子。在这项研究中，两种输送方法（输液泵和局部应用）被用于输送重组人促红细胞生成素（rHuEPO）以促进皮肤再生。首先，通过输液泵将rHuEPO给予小鼠深部的二级烫伤部位。 rHuEPO泵组的血管化明显高于对照组。其次，在大鼠的深部二级烫伤部位给予了rHuEPO凝胶的局部局部应用。组织学分析表明，rHuEPO凝胶治疗组的上皮形成率明显高于对照组。免疫组织化学研究表明，rHuEPO凝胶处理组的皮肤再生因子表达明显高于对照组。到目前为止，由于检测这种细血管的技术困难，尚未开发出用于在目标区域中可视化和量化血管网络的准确方法。在这项研究中使用了一种方法，该方法利用一系列步骤来增强图像，从图像背景中消除噪声并跟踪血管边缘以进行血管分割和量化。使用图像分析方法，我们能够检测参与愈合过程的新生血管（厚度小于500μm）的微血管网络，不仅为生长组织提供营养和氧气，还为生长组织提供必要的生长因子细胞使皮肤完全再生。 rHuEPO治疗组的干细胞标记物（CD？31，CD 90，CD 71和Nestin）的表达更高，它们在伤口愈合过程中为新的毛囊生成以及皮肤再生做出了积极贡献。总的来说，rHuEPO组都泵入全身循环系统，并注入局部损伤区域，促使小鼠和大鼠在烫伤的损伤部位形成新的血管网络，从而显着参与烫伤的愈合过程。这些结果可能导致开发用于烫伤伤口的新疗法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号