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首页> 外文期刊>Drug Design, Development and Therapy >Immunosuppressive effects of the standardized extract of Phyllanthus amarus on cellular immune responses in Wistar-Kyoto rats
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Immunosuppressive effects of the standardized extract of Phyllanthus amarus on cellular immune responses in Wistar-Kyoto rats

机译：紫叶标准化提取物对Wistar-Kyoto大鼠细胞免疫反应的免疫抑制作用

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Abstract: Phyllanthus amarus (family: Euphorbiaceae) is of immense interest due to its wide spectrum of biological activities. In the present study, the standardized 80% ethanol extract of P.?amarus was investigated for its modulatory activity on various cellular immune parameters, including chemotaxis of neutrophils, engulfment of Escherichia coli by neutrophils, and Mac-1 expression, in leukocytes isolated from treatedontreated Wistar-Kyoto rats. The detailed cell-mediated activity of P.?amarus was also investigated, including analysis of the effects on T- and B-cell proliferation and CD4+ and CD8+ T-cell subsets in splenic mononuclear cells, and estimation of serum cytokine production by activated T-cells. The main components of the extract, phyllanthin, hypophyllanthin, corilagin, geraniin, ellagic acid, and gallic acid were identified and quantitatively analyzed in the extracts, using validated reversed-phase high-performance liquid chromatography (HPLC) methods. N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced neutrophils isolated from rats administered with the extract of P.?amarus, at doses ranging from 100 to 400 mg/kg for 14 days, revealed a significant dose-dependent reduction in neutrophil migration (P<0.05). Similar patterns of inhibition were also observed in phagocytic activity and in fMLP-induced changes in expression of β2 integrin polymorphonuclear neutrophils. The results in P.?amarus-treated rats also demonstrated a dose-dependent inhibition of both lipopolysaccharide-stimulated B-cell proliferation and concanavalin A–stimulated T-cell proliferation as compared with sensitized control. At a dose of 400 mg/kg (P<0.01), there was a significant decrease in the (%) expression of CD4+ and CD8+ in splenocytes and in serum cytokines of T helper (Th1) (IL-2 and IFN-γ) and Th2 (IL-4). In conclusion, P.?amarus showed effective immunosuppressive activities in cellular immune response, by various immune regulatory mechanisms, and may be useful for improvement of immune-related disorders.
机译：摘要：由于其广泛的生物活性，Ph兰（家庭为大戟科）备受关注。在本研究中，研究了标准的80％乙醇酵母提取物在各种细胞免疫参数（包括嗜中性粒细胞的趋化性，嗜中性粒细胞吞噬大肠杆菌和Mac-1表达）对各种细胞免疫参数的调节活性。处理/未处理的Wistar-Kyoto大鼠。还研究了疟原虫的详细的细胞介导活性，包括分析对脾单核细胞中T细胞和B细胞增殖以及CD4 +和CD8 + T细胞亚群的影响，并通过活化T估计血清细胞因子的产生。 -细胞。使用经过验证的反相高效液相色谱（HPLC）方法，对提取物中的主要成分叶绿素，次生叶黄素，丝兰胶，香叶素，鞣花酸和没食子酸进行了鉴定和定量分析。 N-甲酰基-甲硫酰基-亮氨酰-苯丙氨酸（fMLP）诱导的嗜中性粒细胞，从服用P.amarus提取物的大鼠中分离，剂量范围为100至400 mg / kg，共14天，显示出明显的剂量依赖性降低中性粒细胞迁移（P <0.05）。在吞噬活性和fMLP诱导的β2整联蛋白多形核中性粒细胞表达变化中也观察到了类似的抑制模式。与致敏对照相比，经P.amarus处理的大鼠的结果还显示出剂量依赖性抑制脂多糖刺激的B细胞增殖和伴刀豆球蛋白A刺激的T细胞增殖。在400 mg / kg的剂量下（P <0.01），脾脏细胞和T辅助细胞（Th1）（IL-2和IFN-γ）的血清细胞因子中CD4 +和CD8 +的表达百分比（％）显着降低。和Th2（IL-4）。总之，P.amarus通过各种免疫调节机制在细胞免疫反应中显示出有效的免疫抑制活性，可能对改善免疫相关疾病有用。

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