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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Host-Specific Toxin Production by the Tomato Target Leaf Spot Pathogen Corynespora cassiicola
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Host-Specific Toxin Production by the Tomato Target Leaf Spot Pathogen Corynespora cassiicola

机译：番茄靶叶病原菌Corynespora cassiicola产生宿主特异性毒素

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摘要

The culture filtrate (CF) of Corynespora cassiicola, the causal agent of target leaf spot of tomato, produced visible small, pinpoint, water-soaked lesions on the lower surface of wounded, detached leaves after incubation for 48 h. The extraction, isolation and purification of C. cassiicola tomato (CCT) toxin was achieved by anion exchange and gel filtration chromatography. Treatments of the CF with Diaion HP-20 resin and Cosmosil C18-OPN gel were found to be highly effective. The toxin purified partially by cosmosil gel filtration was highly active against the tomato cultivar Momotaro used in the bioassay test, and the fraction eluted with 50% methanol showed toxicity only on the tomato plant. However, in the bioassay of the fractions after Sephadex LH-20 gel filtration no toxicity could be detected. Moreover, methanol fractions (40% and 60%) of the culture filtrate extracted by Sep-Pak cartridge were subjected to high performance liquid chromatography systems. The bioactive fraction (40% methanol) selected for analysis was eluted as double peaks. The results showed that C. cassiicola produced a host-specific toxin during colonization of tomato leaves and disease incidence.
机译：番茄目标叶斑病的病原体Corynespora cassiicola的培养滤液（CF）孵育48小时后，在受伤的，离去的叶片下表面产生了可见的细小，尖锐的，浸水的病灶。通过阴离子交换和凝胶过滤色谱法可对C. cassiicola Tomato（CCT）毒素进行提取，分离和纯化。发现用Diaion HP-20树脂和Cosmosil C18-OPN凝胶处理CF非常有效。通过cosmosil凝胶过滤部分纯化的毒素对生物测定测试中使用的番茄栽培种桃太郎具有很高的活性，用50％甲醇洗脱的级分仅对番茄具有毒性。然而，在Sephadex LH-20凝胶过滤后的级分的生物测定中，未检测到毒性。此外，将通过Sep-Pak柱提取的培养滤液的甲醇馏分（40％和60％）进行高效液相色谱分析。选择用于分析的生物活性部分（40％甲醇）洗脱为双峰。结果表明，C。cassiicola在番茄叶片定植和疾病发生期间产生了宿主特异性毒素。

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