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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Effect of Tissue on the Inoculation and Detection of Xylella fastidiosa in the Grapevine
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Effect of Tissue on the Inoculation and Detection of Xylella fastidiosa in the Grapevine

机译：组织对葡萄中小木鼠接种和检测的影响

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摘要

Pierce's disease of the grapevine is caused by a xylem-limited bacterium, Xylella fastidiosa (Xf). Susceptible vines die within a few years after infection. However, the mechanism of pathogenesis is not fully understood. It is very important to optimize the timing and positioning of inoculation in host plants to study the infection process. Rooted dormant cuttings of cv. Chardonnay (susceptible vine) were grouped as stem tissue hardening and shoot elongation for needle-inoculation with 20 μl of 2 x 108 cfu ml-1 of Xf suspension. The infected plants were sampled 2 and 3 weeks following postinoculation (wpi) at 10 cm above the point of inoculation. Negative controls were prepared with 5 plants from each group with water inoculation. DAS-ELISA was used to detect Xf. Plants with soft stem tissue were not well suited to needle inoculation due to their poorly hardened stems, and bacterial suspension could not be fully supplied to the tissue. However, ELISA worked well with both stem types. Chardonnay cuttings with ?30 cm long semi-hardwood tissue, sampled 2 wpi, resulted in fast bacterial movement, while ELISA readings dramatically dropped 3 wpi. Samples from the apical end of inoculated shoots had low ELISA readings, likely the result of young xylem development.
机译：葡萄皮尔斯氏病是由木质部限制细菌Xylella fastidiosa（Xf）引起的。易感染的葡萄藤在感染后的几年内死亡。但是，发病机理尚不完全清楚。优化宿主植物中接种的时间和位置对研究感染过程非常重要。植根休眠的s插。霞多丽（易感藤蔓）分为茎组织变硬和枝条伸长，以便用20μl2 x 108 cfu ml-1 Xf悬浮液进行针头接种。接种后2周和3周后在高于接种点10cm处对感染的植物取样。用水接种的每组5株植物制备阴性对照。使用DAS-ELISA检测Xf。具有软茎组织的植物由于茎变硬而不能很好地适合于针头接种，并且细菌悬浮液无法完全提供给组织。但是，ELISA对两种茎类型均适用。霞多丽插条长约30厘米的半硬木组织，采样2 wpi，可快速移动细菌，而ELISA读数则急剧下降3 wpi。接种枝条顶端的样品的ELISA读数较低，这很可能是木质部木质部发育的结果。

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