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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Somatic Embryogenesis from Immature Cotyledons of Some European Chestnut (Castanea sativa Mill.) Cultivars
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Somatic Embryogenesis from Immature Cotyledons of Some European Chestnut (Castanea sativa Mill.) Cultivars

机译：一些欧洲板栗（Castanea sativa Mill。）品种未成熟子叶的体细胞胚发生

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摘要

In 4 European chestnut (Castanea sativa Mill.) cultivars ('Hac?ibi?', 'Karamehmet', 'Osmano?lu' and 'Sar?a?lama'), somatic embryogenesis from immature cotyledons of open-pollinated seeds collected 5, 6, 7, and 8 weeks after anthesis and cultured on Driver and Kuniyuki walnut (DKW) medium was determined in this study. The percentage of embryogenic cotyledons and the number of embryos per embryogenic cotyledon were recorded at the end of the second subculture. The results showed that the highest percentage of embryogenesis ranged from 40% to 70% and the number of embryos per embryogenic cotyledon varied from 4.04 to 7.70 among the cultivars. Cotyledons collected 5, 6, and 7 weeks after anthesis were the most embryogenic
机译：在4个欧洲板栗（Castanea sativa Mill。）品种中（'Hac？ibi？'，'Karamehmet'，'Osmano？lu'和'Sar？a？lama'），从未授粉种子的未成熟子叶中获得体细胞胚发生5本研究确定了在开花后第6、7、8周并在Driver和Kuniyuki胡桃（DKW）培养基上进行培养。在第二次传代培养结束时记录胚性子叶的百分比和每个胚性子叶的胚胎数。结果表明，在各品种中，最高的胚发生率在40％至70％之间，每个胚性子叶的胚数在4.04至7.70之间。花后5、6和7周收集的子叶是最易胚芽的

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